| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 264,098 | G→T | L50L (CTC→CTA) | ykfH ← | uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 264,098 | 0 | G | T | 94.4% | 40.4 / NA | 18 | L50L (CTC→CTA) | ykfH | uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (5/12); minor base A (1/0); total (6/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTAC > NC_000913/264034‑264150 | gggTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTTTACCGCAAgg > 2:873404/1‑68 (MQ=255) gTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc > 1:642494/1‑68 (MQ=255) gATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTc > 1:797469/1‑68 (MQ=255) cTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGc < 2:675899/68‑1 (MQ=255) cTCATCAGGCAGGTATAGGGCCCAGTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGc < 1:405475/68‑1 (MQ=255) catcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGa < 1:551572/68‑1 (MQ=255) caggcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGatat < 2:377261/68‑1 (MQ=255) ataGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGt > 1:758731/1‑68 (MQ=255) ataGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGt > 2:522389/1‑68 (MQ=255) ataGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATAACGCTAGGACTCCGGCGATATCCGGCACGt > 1:467304/1‑68 (MQ=37) cATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCg < 2:198282/68‑1 (MQ=255) aTTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCgg < 1:666368/68‑1 (MQ=255) tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGTCGATATCCGGAACGTCCCTGCCGgtg < 2:297203/68‑1 (MQ=21) tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACGCCGGCGATATCCGGAACGTCCCTGCCGgtg < 1:265076/68‑1 (MQ=21) tGCGGTCCTGGCGGCGTGCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg < 1:656467/68‑1 (MQ=255) cggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGAc < 1:607442/68‑1 (MQ=255) cggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGAc < 1:752702/68‑1 (MQ=255) ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTAc < 1:576931/68‑1 (MQ=255) | GGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTAC > NC_000913/264034‑264150 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |