Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,679,525	G→T	A1074D (GCT→GAT)	yphG ←	DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,679,525	0	G	T	100.0%	180.9 / NA	54	A1074D (GCT→GAT)	yphG	DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (32/22);  total (32/22)

GGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCG  >  NC_000913/2679460‑2679588                                                                  |                                                                gggTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATcc                                                               >  1:732819/1‑68 (MQ=255) gggTTTAGTGAACGCGGTTAAAAATAAAAGGCATAACGGTGGTGAATAATGCCGCTTTCGGCCAATcc                                                               >  1:827731/1‑68 (MQ=255)   gTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCgg                                                             >  2:605417/1‑68 (MQ=255)   gTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCgg                                                             >  2:915200/1‑68 (MQ=255)   gTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCgg                                                             >  1:792079/1‑68 (MQ=255)    tttAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGa                                                            <  2:749447/68‑1 (MQ=255)     ttAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGAt                                                           >  1:283735/1‑68 (MQ=255)        gTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATACGGATTaa                                                        >  2:948474/1‑68 (MQ=255)         tGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAAt                                                       >  1:30871/1‑68 (MQ=255)         tGAACGCGGTTAAAAATAAAAGGCATCAAGGTGGTGAATAATGCCGCTTTCGGCAAATCCGGATTAAt                                                       >  2:734214/1‑68 (MQ=255)            aCGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGc                                                    <  1:744512/68‑1 (MQ=255)             cgcgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGcc                                                   >  1:412129/1‑68 (MQ=255)              tctgTTAAAAATAATAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCg                                                  <  1:69632/65‑1 (MQ=255)              gcgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCg                                                  >  2:356426/1‑68 (MQ=255)              gcgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCg                                                  <  2:732822/68‑1 (MQ=255)              gcgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCg                                                  <  2:773832/68‑1 (MQ=255)                 gTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCa                                               >  1:512337/1‑68 (MQ=255)                  ttAAAAATAAAAGGCATCACGGTGGTTAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCaa                                              <  2:876173/68‑1 (MQ=255)                        aTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAgc                                        >  2:354894/1‑68 (MQ=255)                        aTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAga                                        >  1:222371/1‑67 (MQ=255)                        aTAAAAGGCATCACGGTGGTGAATAATGCAGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAgc                                        >  2:411662/1‑68 (MQ=255)                        aTAAAAGGCATCACGGTGGTGAATAATGCAGCTTTCGGACAATCCGGATTAATCGCCGTCAATTCAgc                                        >  2:438816/1‑68 (MQ=255)                             aGGCATCACGGTGGTGTATAATGCCGCTTTCGGCCGATCCGGATTAATCGCCGTCGATTCAGCGCGAg                                   >  2:122451/1‑68 (MQ=255)                             aGGAATAACGGTGGTGAATAATGCCGCTTTCGACCAATCCGGAATAATCGCCGTCAAATCAGCGCGAg                                   >  1:157382/1‑68 (MQ=255)                                 aTCACNGNGGTGAATAATGCCTCTTTCGGCCAATCCTGATTAATCGCCGTCAATTCAGCGCGAGCAGa                               <  2:157336/68‑1 (MQ=255)                                 aTCACGTTTGTGAATAATGCCGCTTTCTGCCAATCCGTATTAATCGCCGGCAATTCAGCTCGAGCAgt                               <  2:222371/68‑2 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                               <  2:301890/68‑1 (MQ=255)                                   ctcGGTGTTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                             <  2:178658/66‑1 (MQ=255)                                   cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                             <  2:556002/68‑1 (MQ=255)                                   cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                             <  2:512340/68‑1 (MQ=255)                                   cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                             <  1:209118/68‑1 (MQ=255)                                   cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                             <  1:77281/68‑1 (MQ=255)                                   cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                             <  1:915196/68‑1 (MQ=255)                                     cGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCa                           <  2:565497/68‑1 (MQ=255)                                              ataatGCCGCTTTCGGCCAATCCGGATTAATCGCCg                                                  >  1:319568/1‑36 (MQ=255)                                              ataatGCCGCTTTCGGCCAATCCGGATTAATCGCCg                                                  <  2:319571/36‑1 (MQ=255)                                              ataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa                  >  2:597259/1‑68 (MQ=255)                                              ataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa                  >  2:794412/1‑68 (MQ=255)                                              ataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCAAATTGCGCa                  >  1:924046/1‑68 (MQ=255)                                              ataatGCCGATTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa                  >  1:803586/1‑68 (MQ=255)                                                aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCCAc                >  2:739228/1‑68 (MQ=255)                                                aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc                <  1:596863/68‑1 (MQ=255)                                                  tGCCGCTTTCGGCCAATCCGGATTAATCGCCGGCAATTCAGCGCGAGCAGACTCATATTGCGCAACCt              <  2:886327/68‑1 (MQ=255)                                                   gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCTATTCAGCGCGAGCAGACTCATATTGCGCAACCTc             >  2:457507/1‑68 (MQ=255)                                                   gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTc             >  2:809710/1‑68 (MQ=255)                                                   gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTc             >  2:339168/1‑68 (MQ=255)                                                     cGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTccc           >  2:431235/1‑68 (MQ=255)                                                     cGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTccc           <  2:412132/68‑1 (MQ=255)                                                            ggCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTcc    <  2:665347/68‑1 (MQ=255)                                                             gCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTccc   >  1:804411/1‑68 (MQ=255)                                                              ccAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATAtt                       <  1:378844/47‑1 (MQ=255)                                                              ccAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATAtt                       >  2:378847/1‑47 (MQ=255)                                                              ccAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAGCCTCCCCGAGTCCcg  >  2:346678/1‑68 (MQ=255)                                                              ccAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCAcg  >  2:930198/1‑68 (MQ=255)                                                                  |                                                                GGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCG  >  NC_000913/2679460‑2679588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑