Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,413,571	T→C	intergenic (‑106/‑293)	acrS ← / → acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,413,571	0	T	C	100.0%	53.1 / NA	15	intergenic (‑106/‑293)	acrS/acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/7);  total (8/7)

TAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAATAT  >  NC_000913/3413512‑3413631                                                            |                                                             tAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCg                                                      >  2:362439/1‑68 (MQ=255)               cTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTaaataaat                                            <  1:501487/64‑1 (MQ=255)               cTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTaaataaat                                            >  2:501490/1‑64 (MQ=255)               cTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAataata                                        >  1:142901/1‑68 (MQ=255)                                tttATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAg                       >  2:126279/1‑68 (MQ=255)                                         gagGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCa              <  2:188371/68‑1 (MQ=255)                                              tAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACa         <  2:146757/68‑1 (MQ=255)                                                  atTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACa         >  1:156171/1‑64 (MQ=255)                                                  atTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACa         <  2:156171/64‑1 (MQ=255)                                                  atTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCaa     >  2:939570/1‑68 (MQ=255)                                                     gAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAatat  <  1:126279/68‑1 (MQ=255)                                                      aGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATAc                   >  1:306259/1‑50 (MQ=255)                                                      aGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATAc                   <  2:306261/50‑1 (MQ=255)                                                      aGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACAt                 >  1:149341/1‑52 (MQ=255)                                                      aGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACAt                 <  2:149341/52‑1 (MQ=255)                                                            |                                                             TAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAATAT  >  NC_000913/3413512‑3413631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑