Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,215	Δ1 bp	coding (446/531 nt)	mprA →	transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,215	0	A	.	100.0%	86.8 / NA	20	coding (446/531 nt)	mprA	transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (15/5);  total (15/5)

ACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGG  >  NC_000913/2811156‑2811275                                                            |                                                             accaccGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGa                                                     <  2:498442/68‑1 (MQ=255) accaccGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGa                                                     >  2:411702/1‑68 (MQ=255) accaccGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGa                                                     >  2:162173/1‑68 (MQ=255)  cccccGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAg                                                    >  2:100609/4‑68 (MQ=255)   caccGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGc                                                   >  1:326988/1‑68 (MQ=255)               aCTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCa                                       >  2:422530/1‑68 (MQ=255)               aCTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCa                                       >  1:16949/1‑68 (MQ=255)               aCTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCa                                       >  2:328181/1‑68 (MQ=255)                 tgcctgcATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCaaa                                     >  2:425372/1‑68 (MQ=255)                   cctgcATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCATATCGCCCGCAAAtt                                   >  2:55070/1‑68 (MQ=255)                   cctgcATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAAtt                                   <  1:328182/68‑1 (MQ=255)                             ctctGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGt                         <  2:165444/68‑1 (MQ=255)                             ctctGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGt                         >  1:680069/1‑68 (MQ=255)                             ctctGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCACTCCCGt                         >  1:525199/1‑68 (MQ=255)                                 ggTCCGCGCTCAGCACAAAAGAAAAAG‑TAAGCTAGAGCAAATCACCCGCAAATTGCTCTAACGTCTCg                     >  2:504115/1‑68 (MQ=255)                                   tCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGAc                   >  2:125337/1‑68 (MQ=255)                                      gcgcTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAg                <  2:16949/68‑1 (MQ=255)                                        gcTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGAt              >  1:398160/1‑68 (MQ=255)                                           cAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGa           <  2:674906/68‑1 (MQ=255)                                                    aGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACgg  >  1:630625/1‑68 (MQ=255)                                                            |                                                             ACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGG  >  NC_000913/2811156‑2811275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑