| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | 2548123 = | 26 (1.180) | 3 (0.160) | 3/114 | 1.8 | 12.4% | coding (22/1305 nt) | yfeW | penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity |
| ? | NC_000913 | 2548199 = | 20 (1.050) | coding (98/1305 nt) | yfeW | penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity | |||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
TTCACCACGCTCTTTGGCTGCCGTAACGTCAATCTGGACTGATATCATGAAACGGACAATGCTCTATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGCGCCGCAAAATACCCTGTTCTGACAGAAAGCTCGCC > NC_000913/2548056‑2548190 | taaccgacatcaacttgctggctaatccagcgatccatctggttaagccgttcgactttaatccctgc > 2:83657‑M1/68‑68 (MQ=255) ccgacatcaacttgctggctaatccagcgatccatctggttaagccgttcgacgttaaaccctgCttt < 2:790893‑M1/4‑1 (MQ=255) ccacGCGCTTTGGCTGCCGTAACGTCAATCTGGACTGATATCATGAAACGGACAATGCTCTATCTTtc < 2:71095/68‑1 (MQ=255) acGCTCTTTGGCTGCCGTAACGTCAATCTGGACTGATATCATGAAACGGACAATGCTCTATCTTtctc < 1:19576/68‑1 (MQ=255) gCTCTTTGGCTGCCGTAACGTCAATCTGGACTGATATCATGAAACGGACAATGCTCTATCTTTCTctg < 1:801089/68‑1 (MQ=255) atcaacttgctggctaatccagcgatccatctggttaagccgttcgacgttaaaccctgCTTTCTctg > 2:382449‑M1/60‑68 (MQ=255) acttgctggctaatccagcgatccatctggttaagccgttcgacgttaaaccctgc < 1:542163‑M1/1‑1 (MQ=255) acttgctggctaatccagcgatccatctggttaagccgttcgacgttaaaccctgc > 2:542163‑M1/56‑56 (MQ=255) gctggctaatccagcgatccatctggttaagccgttcgacgttaaaccctgCTTTCTCTGCTGGCTGt > 1:27594‑M1/52‑68 (MQ=255) gCTGCCGTAACGTCAATCTGGACTGATATCATGAAACGGACAATGCTCTATCTTTCTCTGCTGGCTGt > 1:148158/1‑68 (MQ=255) gCTGCCGTAACGTCAATCTGGACTGATATCATGAAACGGACAATGCTCTATCTTTCTCTGCTGGCTGt > 2:750649/1‑68 (MQ=255) ccGTAACGTCAATCTGGACTGATATCATGAAACGGACAATGCTCTATCTTTCTCTGCTGGCTGTTAGt < 1:750649/68‑1 (MQ=255) aaCGTCAATCTGGACTGATATCATGAAACGGACAATGCTCTATCTTTCTCTgctgg > 1:177318/1‑56 (MQ=255) aaCGTCAATCTGGACTGATATCATGAAACGGACAATGCTCTATCTTTCTCTgctgg < 2:177318/56‑1 (MQ=255) tCTGGACTGATATCATGAAACGGACAATGCTCTATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAgc > 2:402970/1‑68 (MQ=255) tCTGGACTGATATCATGAAACGGACAATGCTCTATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAgc < 1:88673/68‑1 (MQ=255) tCTGGACTGATATCATGAAACGGACAATGCTCTATCTTTCTCTGCTGGCTGTTAGTTGTAGCGGCAgc < 1:154999/68‑1 (MQ=255) cTGGACTGATATCATGAAACGGACAATGCTCTATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAgcg > 2:526966/1‑68 (MQ=255) aCTGATATCATGAAACGGACAATGCTCTATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGcgccgc < 1:163324/68‑1 (MQ=255) aCTGATATCATGAAACGGACAATGCTCTATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGcgccgc > 2:74040/1‑68 (MQ=255) aCTGATATCATGAAACGGACAATGCTCTATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGcgccgc > 1:219104/1‑68 (MQ=255) aCTGATATCATGAAACGGACAATGCTCTATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGcgccgc < 1:526385/68‑1 (MQ=255) ctggttaagccgttcgacgttaaaccctgCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGCGCCGCa < 2:167147‑M1/39‑1 (MQ=255) tggttaagccgttcgacgttaaaccctgCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGCGCCGCaa > 1:167147‑M1/29‑68 (MQ=255) tGATATCATGAAACGGACAATGCTCTATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGCGCCGCaa > 2:761310/1‑68 (MQ=255) tGATATCATGAAACGGACAATGCTCTATCTTTCTCTGATGGCTGTTAGTTGTAGCGTCAGCGCCGCaa < 1:87001/68‑1 (MQ=255) tatCATGAAACGGACAATGCTCTATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGCGCCGCAAAAt > 2:511890/1‑68 (MQ=255) tatCATGAAACGGACAATGCTCTATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGCGCCGCAAAAt > 2:203857/1‑68 (MQ=255) aaCGGACAATGCTCTATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGCGCCGCAAAATACCCTGtt > 2:303749/1‑68 (MQ=255) aaCGGACAATGCTCTATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGCGCCGCAAAATACCCTGtt > 2:302744/1‑68 (MQ=255) cGGACAATGCTCTATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGCGCCGCAAAATACCCTGTTCt < 1:402971/68‑1 (MQ=255) aTGCTCTATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGCGCCGCAAAATACCCTGTTCTGACAGa > 1:375203/1‑68 (MQ=255) ctctATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGCGCCGCAAAAt < 2:554195/49‑1 (MQ=255) ctctATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGCGCCGCAAAAt > 1:554195/1‑49 (MQ=255) CTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGCGCCGCAAAATACCCTGTTCTGACAGAAAGCTCGcc > 2:219079‑M1/1‑68 (MQ=255) CTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGCGCCGCAAAATACCCTGTTCTGACAGAAAGCTCGcc < 1:219080‑M1/68‑1 (MQ=255) | TTCACCACGCTCTTTGGCTGCCGTAACGTCAATCTGGACTGATATCATGAAACGGACAATGCTCTATCTTTCTCTGCTGGCTGTTAGTTGTAGCGTCAGCGCCGCAAAATACCCTGTTCTGACAGAAAGCTCGCC > NC_000913/2548056‑2548190 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |