Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	264,098	0	G	T	96.0%	74.4 / NA	25	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (9/15);  minor base A (1/0);  total (10/15)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCC  >  NC_000913/264034‑264156                                                                 |                                                           gggTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAgg                                                         <  2:715774/68‑1 (MQ=255)   gTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc                                                       >  1:402483/1‑68 (MQ=255)   gTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc                                                       >  1:631241/1‑68 (MQ=255)    tGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGGTCGGCGTATACCGCTAGGACt                                                      <  2:552603/68‑1 (MQ=255)     gATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCAAGGCCTc                                                     >  2:743832/1‑68 (MQ=255)        gCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCgg                                                  >  1:52966/1‑68 (MQ=255)        gCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCgg                                                  >  2:277313/1‑68 (MQ=255)              caggcaggTATAGGGCCCTTTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGatat                                            <  1:1029/68‑1 (MQ=255)              caggcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGatat                                            <  2:34372/68‑1 (MQ=255)                    ggTATAGGGCCAATTGCGGTCCAGGCGGCGTACGGCGTATAACGCTAGGACTCCGGCGATAACCGGaa                                      >  1:804967/1‑68 (MQ=255)                       ataGGGCCCATTGCGGTCCTGGCTGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGt                                   <  2:268380/68‑1 (MQ=37)                        taGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGTTATCCGGAACGTc                                  <  2:441314/68‑1 (MQ=255)                           ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCt                               <  2:519055/68‑1 (MQ=255)                            gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                              >  1:768012/1‑68 (MQ=255)                            gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                              >  1:434108/1‑68 (MQ=255)                            gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                              >  2:506576/1‑68 (MQ=255)                                 ttGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt                         <  2:118477/68‑1 (MQ=255)                                  tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                        >  2:360242/1‑68 (MQ=255)                                  tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                        <  2:458867/68‑1 (MQ=255)                                  ggCGGGCCTGGCGGCGTTCGTCGTATACCGCTAGGACGCCGGCGATATCCGGAACGTCCCTGCCgggg                        <  1:354006/67‑3 (MQ=255)                                           ggcggcGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAg               <  2:768013/68‑1 (MQ=255)                                             cggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGAc             <  1:431734/68‑1 (MQ=255)                                                cgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCt          <  2:39164/68‑1 (MQ=255)                                                   tCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTAcc       <  1:716112/68‑1 (MQ=255)                                                        gTATACCGCTAGGACGCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGcc  <  2:705877/68‑1 (MQ=255)                                                                 |                                                           GGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCC  >  NC_000913/264034‑264156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑