Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,215	Δ1 bp	coding (446/531 nt)	mprA →	transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,215	0	A	.	100.0%	134.1 / NA	30	coding (446/531 nt)	mprA	transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (20/10);  total (20/10)

ACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGT  >  NC_000913/2811156‑2811276                                                            |                                                              accaccGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGa                                                      >  1:668640/1‑68 (MQ=255)   caccGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGc                                                    >  1:146950/1‑68 (MQ=255)            aTAGCTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCAccc                                           >  1:210626/1‑68 (MQ=255)               aCTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCa                                        >  2:24919/1‑68 (MQ=255)               aCTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCa                                        <  2:212748/68‑1 (MQ=255)               aCTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCa                                        >  2:122121/1‑68 (MQ=255)                 tgcctgcATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCaaa                                      >  2:974907/1‑68 (MQ=255)                   cctgcATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAAtt                                    <  1:143727/68‑1 (MQ=255)                       cATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGctc                                >  1:226700/1‑68 (MQ=255)                        aTCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGctct                               <  1:309430/68‑1 (MQ=255)                          cAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTcc                             <  1:214914/68‑1 (MQ=255)                             ctctGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGt                          >  2:319349/1‑68 (MQ=255)                              tctGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGtc                         >  1:718108/1‑68 (MQ=255)                              actGGTCCTCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACACGCAAATTGCACTCACGta                         >  2:250951/2‑67 (MQ=255)                                 ggTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCg                      >  2:835648/1‑68 (MQ=255)                                   tCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGAc                    >  2:615176/1‑68 (MQ=255)                                   tCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGAc                    >  2:519355/1‑68 (MQ=255)                                   tCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGACTCGAc                    >  2:45363/1‑68 (MQ=255)                                   tCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAAATGCTCTCCCGTCTCGAc                    >  2:520668/1‑68 (MQ=255)                                        gcTCAGCACAACAGAGAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGAt               >  1:368739/1‑68 (MQ=255)                                           cAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGa            <  1:615171/68‑1 (MQ=255)                                           cAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGa            <  2:742919/68‑1 (MQ=255)                                             gCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAAc          >  1:636965/1‑68 (MQ=255)                                                caacaGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAg       <  2:19089/68‑1 (MQ=255)                                                    aGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGa            >  2:12494/1‑59 (MQ=255)                                                    aGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGa            <  1:12494/59‑1 (MQ=255)                                                    aGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACgg   >  2:354113/1‑68 (MQ=255)                                                    aGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACgg   >  2:541564/1‑68 (MQ=255)                                                     gAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGgt  <  2:247134/68‑1 (MQ=255)                                                     gAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATAGCTCTCCCGTCTCGACCAGATGGAACAAGACGgt  <  2:85273/68‑1 (MQ=255)                                                            |                                                              ACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGT  >  NC_000913/2811156‑2811276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑