| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 86,365 | 0 | G | C | 40.0% | 13.8 / 11.1 | 20 | A246P (GCG→CCG) | ilvI | acetolactate synthase 3 large subunit |
| Reads supporting (aligned to +/- strand): ref base G (6/6); new base C (0/8); total (6/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATA > NC_000913/86308‑86431 | aaagaaaCGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGCCGTTTCCGGc < 1:828534/68‑1 (MQ=255) gaacCGGTGGAGGCGTTGAATCTGCCCGTCGTTTGCTCATTGATGGGGCTGGGGCCGTTTCCGGCAAc < 1:986956/68‑1 (MQ=255) gaaaCGGTGGAGGCGATGAATCTGCACGTTGTTTGCTAATTGATGGGGCTGGGGGCGTTTCCGGaaaa > 2:845609/1‑67 (MQ=255) cGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCAt < 2:548898/68‑1 (MQ=255) tGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGt < 2:694851/68‑1 (MQ=255) tGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGCCGTTTCCGGCAACGCATCGt < 2:661092/68‑1 (MQ=255) ggCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAgg > 2:897416/1‑68 (MQ=255) gCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGCCCTTTCCGGCAACGCATCGTCAGGc < 2:1039617/68‑1 (MQ=255) tGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGCCGTTTCCGGCAACGCATCGTCAGGCACTg < 2:899959/68‑1 (MQ=255) gAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGCCGTTTCCGGCAACGCATCGTCAGGCACTgg < 2:600725/68‑1 (MQ=255) aaTCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTggg > 1:806703/1‑68 (MQ=255) aaTCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTggg > 1:1023018/1‑68 (MQ=255) aaTCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGCCGTTTCCGGCAACGCATCGTCAGGCACTggg < 1:213886/68‑1 (MQ=255) aaTCTGCCCCGTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTggg < 1:215000/68‑1 (MQ=255) tGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATg < 2:834338/68‑1 (MQ=255) ttttGCTCATTGATGGGGCTGGGGCCGTTTCCGGCAACGCATCGTCAGGCTCTGGTCATGCTGTGact < 1:975213/67‑3 (MQ=255) cTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACg > 2:613861/1‑68 (MQ=255) tCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACgg < 1:328854/68‑1 (MQ=255) tGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACg > 2:636203/1‑63 (MQ=255) tGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACg < 1:636198/63‑1 (MQ=255) ggCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAata > 1:780090/1‑68 (MQ=255) | AAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATA > NC_000913/86308‑86431 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |