Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,679,525	G→T	A1074D (GCT→GAT)	yphG ←	DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,679,525	0	G	T	100.0%	98.9 / NA	30	A1074D (GCT→GAT)	yphG	DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/13);  total (17/13)

GGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCC  >  NC_000913/2679460‑2679578                                                                  |                                                      gggTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATcc                                                     >  1:167975/1‑68 (MQ=255)   gTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCgg                                                   >  1:353306/1‑68 (MQ=255)   gTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCgg                                                   >  2:968685/1‑68 (MQ=255)   gTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCgg                                                   >  1:634648/1‑68 (MQ=255)   gTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCgg                                                   >  2:52855/1‑68 (MQ=255)     ttAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGAt                                                 >  1:297137/1‑68 (MQ=255)              tctgTTAAAAATAAAAGTCATCACGTTGGTGATTAATGCCGCTTTCGGCCAATCCGGATTTATCGCCg                                        <  2:537665/65‑1 (MQ=255)              gcgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCg                                        >  1:359060/1‑68 (MQ=255)                        aTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAgc                              >  1:631687/1‑68 (MQ=255)                        aTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAgc                              >  2:53134/1‑68 (MQ=255)                               gCATCTCGGAGGTGAATAATGCCGCTTTCGGCCAGTCCGGATTAATCGCCGTCAAATCAGCGAGAGCa                       >  2:872047/1‑68 (MQ=255)                               gCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCa                       >  2:602396/1‑68 (MQ=255)                                 accACGGTGGTGTATAATGCCGCTTTCGGCCAATCCGGATTATTCGCCGTCAATTCAGCGCGAGCAGa                     <  2:386813/66‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                     <  2:212495/68‑1 (MQ=255)                                   cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                   <  1:593968/68‑1 (MQ=255)                                   cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                   <  2:650822/68‑1 (MQ=255)                                   cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                   <  1:355934/68‑1 (MQ=255)                                   cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                   <  2:979623/68‑1 (MQ=255)                                      ggtggtGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCat                <  2:359063/68‑1 (MQ=255)                                      ggtggtGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCat                <  2:122287/68‑1 (MQ=255)                                              atactGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa        >  1:948937/1‑68 (MQ=255)                                              ataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa        >  2:382839/1‑68 (MQ=255)                                                aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGCCTCATATTGCGCAAc      >  2:53061/1‑68 (MQ=255)                                                aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc      >  2:260882/1‑68 (MQ=255)                                                aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc      <  1:52855/68‑1 (MQ=255)                                                  tGCCGCTTTCGTCCTATCTGGATTTATCGCCGTCAATTTAGCGCGAGCAGACTCATATTGCGCATCCt    <  1:928328/68‑1 (MQ=255)                                                   gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTc   >  2:928944/1‑68 (MQ=255)                                                    ccGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTcc  <  2:148731/68‑1 (MQ=255)                                                        tttCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                   >  1:1028670/1‑47 (MQ=255)                                                        tttCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                   <  2:1028679/47‑1 (MQ=255)                                                                  |                                                      GGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCC  >  NC_000913/2679460‑2679578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑