Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,413,571	T→C	intergenic (‑106/‑293)	acrS ← / → acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,413,571	0	T	C	100.0%	28.0 / NA	9	intergenic (‑106/‑293)	acrS/acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/6);  total (3/6)

AAAATAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAATAT  >  NC_000913/3413508‑3413631                                                                |                                                             aaaaTAGCGAAGGTTAGTCTATCACCTTATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATaa                                                          >  1:66432/1‑68 (MQ=255)                 aTCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAataaataa                                          <  1:375399/68‑1 (MQ=255)                  tCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAaataat                                         >  1:101678/1‑68 (MQ=255)                    tATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAatat                                       >  2:597206/1‑68 (MQ=255)                      tCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAatatat                                     <  2:828840/68‑1 (MQ=255)                         ccTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATAtatt                                  <  1:597201/68‑1 (MQ=255)                               gtgtATTTATACGAGAGGCTAATATTGAGTCGCCATAAATCGTTAAATAAATAATATATATTATTTAc                            <  1:545767/68‑1 (MQ=255)                                             gagTCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCa              <  2:475623/68‑1 (MQ=255)                                                         taGTTGCCATAAATCGCTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAatat  <  2:66433/67‑1 (MQ=39)                                                                |                                                             AAAATAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAATAT  >  NC_000913/3413508‑3413631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑