Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,215	Δ1 bp	coding (446/531 nt)	mprA →	transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,215	0	A	.	100.0%	98.4 / NA	22	coding (446/531 nt)	mprA	transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (9/13);  total (9/13)

CACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGT  >  NC_000913/2811158‑2811276                                                          |                                                              caccGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGc                                                    >  1:767683/1‑68 (MQ=255)    cGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCaaa                                                 <  1:1018919/68‑1 (MQ=255)         caTAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCAcc                                            <  2:526527/68‑1 (MQ=255)             aCTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCa                                        >  1:180734/1‑68 (MQ=255)             aCTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCa                                        >  1:298896/1‑68 (MQ=255)               tgcctgcATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCaaa                                      >  2:1281999/1‑68 (MQ=255)                      aTCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGctct                               <  1:1171390/68‑1 (MQ=255)                      aTCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGctct                               <  1:1298003/68‑1 (MQ=255)                       tCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCtctc                              >  1:252481/1‑68 (MQ=255)                        cAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTcc                             <  2:633574/68‑1 (MQ=255)                           ctctGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGt                          <  1:387177/68‑1 (MQ=255)                                 tCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGAc                    >  2:67488/1‑68 (MQ=255)                                 tCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGAc                    >  1:609480/1‑68 (MQ=255)                                 tCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGAc                    >  1:1298701/1‑68 (MQ=255)                                         cAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGa            <  1:558371/68‑1 (MQ=255)                                         cAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGa            <  1:170557/68‑1 (MQ=255)                                         cAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGa            <  1:1159254/68‑1 (MQ=255)                                         cAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGa            <  1:1101610/68‑1 (MQ=255)                                              caacaGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAg       <  1:538494/68‑1 (MQ=255)                                                  aGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGACTCGACCAGATGGAACAAGACgg   >  1:420128/1‑68 (MQ=255)                                                   gAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGgt  <  1:483148/68‑1 (MQ=255)                                                   gAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGgt  <  1:1282000/68‑1 (MQ=255)                                                          |                                                              CACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGT  >  NC_000913/2811158‑2811276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑