| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,081,353 | 0 | G | C | 37.0% | 38.2 / 14.3 | 28 | intergenic (+540/‑3) | putP/efeU | proline:sodium symporter/ferrous iron permease (pseudogene) |
| Reads supporting (aligned to +/- strand): ref base G (11/6); new base C (0/10); total (11/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.05e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGCTATCACTGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGGTGGCATGTTTGTTCCGTTTCTCATTATGTTGCGCGAAGGACTTGAAGCCGCGCTGATTGTCAGTTTGAT > NC_000913/1081289‑1081420 | tCGCTATCACTGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGGTGGCa > 1:836862/1‑68 (MQ=255) tATCACTGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGGTTTCAtgtt < 1:1263998/68‑1 (MQ=255) aCTGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGGTGGCAtgtttgtt > 1:1302190/1‑68 (MQ=255) aCTGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGGTGGCAtgtttgtt > 1:857007/1‑68 (MQ=255) tGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGGTGGCAtgtttgt > 2:737292/1‑65 (MQ=255) tGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGGTGGCAtgtttgt < 1:737294/65‑1 (MQ=255) tGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGGTGGCATGTTTGTTcc > 1:959075/1‑68 (MQ=255) tttACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGGTGGCATGTTTGTTCCGTTtc < 2:1302189/68‑1 (MQ=255) ttACTTTTCATTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGGCCCCATGTTTGTTCCGTTtct < 1:1065994/68‑1 (MQ=255) tACTTTTCTTTGCATAGATTGACTCAGTAAAACGTTTAAGGGTGGGTCCCATGTTTGTTCCGTTtctc < 1:959796/68‑1 (MQ=255) tACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGGTCGCATGTTTGTTCCGTTtctc < 1:1239956/68‑1 (MQ=255) tACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGGCCGCATGTTTGTTCCGTTtctc < 1:170202/68‑1 (MQ=255) cTTTGCATAGATGGACTCAGAAAAACGTTTAAGGGTGGGCCGCATGTTTGTTCCGTTTCTCATTATGt < 1:438814/68‑1 (MQ=255) aTAGATTGACTCAGAAAAACGTTTAAGGGTGGGTGGCATGTTTGTTCCGTTTCTCATTATGCTGcgcg > 2:313243/1‑68 (MQ=255) aTTGACTCAGAAAAACGTTTAAGGGTGGGTGGCATGTTTGTTCCGTTTCTCATTATGTTGCGCGAAgg < 2:188452/68‑1 (MQ=255) ttGACTCAGAAGAGCGTTTAAGGGTGGGTGGCATGTTTGTTCCGTTTCACATGATGTTGCGCGAAggg > 2:939973/1‑67 (MQ=255) ttGACTCAGAAAAACGTTTAAGGGTGGGTGGCATGTTTGTTCCGTTTCTCATTATGTTGCGCGAAGGa > 1:886878/1‑68 (MQ=255) ttGACTCAGAAAAACGTTTAAGGGTGGGTGGCATGTTTGTTCCGTTTCTCATTATGTTGCGCGAAGGa > 2:191982/1‑68 (MQ=255) ttGACTCAGAAAAACGTTTAAGGGTGGGTGGCATGTTTGTTCCGTTTCTCATAATGTTGCGCGAAGGa > 2:133271/1‑68 (MQ=255) tGACTCAGAAAAACGTTTAAGGGTGGGTGGCATGTTTGTTCCGTTTCTCATTATGTTGCGCGAAGGAc < 1:31866/68‑1 (MQ=255) tGACTCAGAAAAACGTTTAAGGGTGGGTCGCATGTTTGTTCCGTTTCTCATTATGTTGCGCGAAGGAc < 2:596730/68‑1 (MQ=255) tGACTCAGAAAAACGTTTAAGGGTGGGCCGCATGTTTGTTCCGTTTCTCATTATGTTGCGCGAAGGAc < 1:311347/68‑1 (MQ=255) cGTTTAAGGGTGGGTGGCATGTTTGTTCCGTTTCTCATTATGTTGCGCGAAGGACTTGAAGCCGCGCt < 1:133271/68‑1 (MQ=255) tttAAGGGTGGGTCGCATGTTTGTTCCGTTTCTCATTATGTTGCGCGAAGGACTTGAAGCCGCGCTGa < 1:153840/68‑1 (MQ=255) tttAAGGGTGGGTCGCATGGTTGTTCCGTTTCTCATTATGTTGCGCGAAGGACTTGAAGCCGCGCTGa < 1:921897/68‑1 (MQ=255) tAAGGGTGGGTCGCATGTTTGTTCCGTTTCTCATTATGTTGCGCGAAGGACTTGAAGCCGCGCTGAtt < 1:870036/68‑1 (MQ=255) aGGGTGGGTGGCATGTTTGTTCCGTTTCTCATTATGTTGCGCGAAGGACTTGAAGCCGCGCTGATTGt < 1:247896/68‑1 (MQ=255) gggagggtGGCATGTTTGTTCCGAATCTCATTATGTTGAGCGAAGGACTTGAAGCCGCGCAGATTGTc > 2:1297409/1‑68 (MQ=255) ggtgggtgGCATGTTTGTTCCGTTTCTCATTATGTTGCGCGAAGGACTTGAAGCCGCGCTGATTGTCa > 2:304599/1‑68 (MQ=255) ggtggCATGTTTGTTCCGTATCTCATTATGTTGCGCGAAGGACTTGAAGCCGCGCTGATTGTCAGttt > 2:1002388/1‑68 (MQ=255) gtggCATGTTTGTTCCGTTTCTCATTATGTTGCGCGAAGGACTTGAAGCCGCGCTGATTGTCa > 1:960205/1‑63 (MQ=255) gtggCATGTTTGTTCCGTTTCTCATTATGTTGCGCGAAGGACTTGAAGCCGCGCTGATTGTCa < 2:960204/63‑1 (MQ=255) gtggCATGTTTGTTCCGTTTCTCATTATGTTGCGCGAAGGACTTGAAGCCGCGCTGATTGTCTGTTTg > 2:424775/1‑68 (MQ=255) ggCATGTTTGATCCGTTTCTCATTATGTTGCGCGAAGGACTTGAAGCCGCGCTGATTGTCAGTTTGAt > 2:807865/1‑68 (MQ=255) | TCGCTATCACTGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGGTGGCATGTTTGTTCCGTTTCTCATTATGTTGCGCGAAGGACTTGAAGCCGCGCTGATTGTCAGTTTGAT > NC_000913/1081289‑1081420 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |