| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,296,060 | 0 | C | T | 40.9% | 42.4 / 87.6 | 66 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base C (16/23); new base T (9/18); total (25/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTA > NC_000913/4295994‑4296123 | cgcAATTGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACAGCAACg < 2:1175116/68‑1 (MQ=255)cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACg < 2:697179/68‑1 (MQ=35) gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGc > 1:923638/1‑68 (MQ=25) gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGc > 2:1155363/1‑68 (MQ=25) gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGc < 2:1068102/68‑1 (MQ=35) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt < 1:521545/68‑1 (MQ=25) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt < 1:494941/68‑1 (MQ=25) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt > 2:521543/1‑68 (MQ=25) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt > 2:494939/1‑68 (MQ=25) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt > 2:528463/1‑68 (MQ=35) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt > 2:483586/1‑68 (MQ=35) aGCCTGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt < 1:250551/68‑1 (MQ=255) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc < 1:156236/68‑1 (MQ=25) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGc > 2:1045965/1‑68 (MQ=35) cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCg > 1:218859/1‑68 (MQ=25) tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGc > 1:1256086/1‑68 (MQ=35) ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCt > 2:259390/1‑68 (MQ=25) ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt > 2:89298/1‑68 (MQ=35) aGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTg < 1:367886/68‑1 (MQ=255) gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcg > 1:49323/1‑68 (MQ=255) gATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgcg < 1:1304995/68‑1 (MQ=255) tttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCt < 1:1052281/68‑1 (MQ=255) tttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCATGCCGCGTCt > 2:951630/1‑68 (MQ=255) ttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCtt < 2:1276567/68‑1 (MQ=255) ttGTAGGGCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCtt < 1:594168/68‑1 (MQ=255) tGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa < 2:1099315/68‑1 (MQ=255) gggTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCa > 2:1192743/2‑68 (MQ=255) aGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCt > 2:1053750/1‑63 (MQ=255) aGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCt < 1:1053751/63‑1 (MQ=255) aGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa > 1:309495/1‑68 (MQ=255) aGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCa > 1:127547/1‑68 (MQ=255) aGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCa > 2:1227979/1‑68 (MQ=255) gTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 2:742968/68‑1 (MQ=255) gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:1211663/68‑1 (MQ=255) aTAAGTCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc < 1:153786/68‑1 (MQ=255) aTAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat < 2:969038/68‑2 (MQ=255) aTAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat < 1:856985/68‑2 (MQ=255) aTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc < 1:483588/68‑1 (MQ=255) aTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc < 2:146423/68‑1 (MQ=255) aTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc < 2:1157939/68‑1 (MQ=255) aTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc < 2:70677/68‑1 (MQ=255) aTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc < 1:973/68‑1 (MQ=255) aTAAGGCGTTTACGCCGCAGCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc < 1:521975/68‑1 (MQ=255) aTAAGGCGCTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc < 2:976381/68‑1 (MQ=255) tAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc < 1:1155364/68‑3 (MQ=255) tAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc > 2:965362/1‑67 (MQ=255) tAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc < 1:965363/67‑1 (MQ=255) tAAGGCGTTTACGCCGCATCCGACAGCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc < 2:654152/68‑3 (MQ=14) tAAGGCGTTTACGCCGCATCCGACAGCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc < 2:1099273/68‑3 (MQ=14) tAAGGCGGTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc < 2:141776/68‑3 (MQ=14) aaGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct < 1:394267/68‑4 (MQ=255) cGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt < 1:1332294/48‑1 (MQ=255) cGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt > 2:1332293/1‑48 (MQ=255) cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACg < 2:1144488/38‑1 (MQ=40) cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACg > 1:1144491/1‑38 (MQ=40) cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACg > 1:1144489/1‑38 (MQ=40) cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACGCCAGa > 2:1242654/1‑68 (MQ=25) tttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa < 1:179427/68‑1 (MQ=255) tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgc < 2:1016903/68‑1 (MQ=255) aCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcg > 2:410769/1‑68 (MQ=255) cgccgcATCCGACTTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcgc < 2:1058243/68‑1 (MQ=255) cgccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGc < 1:283875/58‑1 (MQ=255) cgccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCGACGCCAGACAgcgc < 2:996330/68‑1 (MQ=255) aTCCGACAGCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc < 1:621797/68‑1 (MQ=255) taCATCAACGCCTGATGCGACTCTTGCCGCGTCTTATCTGGCCTACTCCAGCCAGCGCAATAGCCtta < 2:757819/67‑3 (MQ=14) tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa > 1:920891/1‑68 (MQ=39) | CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTA > NC_000913/4295994‑4296123 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |