Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	264,098	0	G	T	97.7%	139.9 / NA	43	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (22/20);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGT  >  NC_000913/264036‑264162                                                               |                                                                 gTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc                                                             >  1:1115576/1‑68 (MQ=255)   gATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTc                                                           >  2:722735/1‑68 (MQ=255)      gCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCgg                                                        >  2:328598/1‑68 (MQ=255)        tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCg                                                      >  2:918037/1‑68 (MQ=255)           tcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGata                                                   >  2:710792/1‑68 (MQ=255)            caggcagtTTTTGTGCCCATTGCGGTCCTGGCGGCGGTCGTCGTATACCGCTAGGACTCCGTCGatat                                                  <  2:245/68‑1 (MQ=255)            caggcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGatat                                                  <  2:743540/68‑1 (MQ=255)            caggcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACGCCGGCGatat                                                  <  2:1278720/68‑1 (MQ=255)             aggcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATc                                                 <  2:661265/68‑1 (MQ=255)                caggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTTGGACTCCGGCGATATCCgg                                              >  1:187646/1‑68 (MQ=255)                caggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCgg                                              <  1:1112014/68‑1 (MQ=255)                caggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCgg                                              >  2:416083/1‑68 (MQ=255)                caggAAAAGGGCACATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCgg                                              >  1:190189/1‑68 (MQ=25)                 aggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGa                                             <  2:224241/68‑1 (MQ=255)                     ataGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGt                                         <  1:1161654/68‑1 (MQ=255)                     ataGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGt                                         >  2:904089/1‑68 (MQ=255)                         ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCt                                     <  2:454999/68‑1 (MQ=255)                         ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCt                                     >  1:647446/1‑68 (MQ=255)                         ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCt                                     >  1:455001/1‑68 (MQ=255)                          gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                    >  2:848873/1‑68 (MQ=255)                          gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                    >  2:1303912/1‑68 (MQ=255)                          gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                    <  2:684289/68‑1 (MQ=255)                               ttGCGGTCCTGGCGGCTTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt                               <  1:1308513/68‑1 (MQ=255)                               ttGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGACCGTCCCTGCCGgt                               >  2:1113430/1‑68 (MQ=255)                               ttGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt                               <  2:82472/68‑1 (MQ=255)                                tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTTGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                              <  2:187646/68‑1 (MQ=255)                                tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                              <  2:1189166/68‑1 (MQ=255)                                tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                              <  1:1052440/68‑1 (MQ=255)                                 gCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtgt                             >  1:894621/1‑68 (MQ=255)                                 gCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtgt                             <  2:366532/68‑1 (MQ=255)                                      ccTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGa                        >  2:1214993/1‑68 (MQ=255)                                         ggcggcGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAg                     <  2:190189/68‑1 (MQ=255)                                           cggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAggc                   >  2:89693/1‑66 (MQ=255)                                           cggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGAc                   >  1:1116503/1‑68 (MQ=255)                                              cgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCt                <  2:1228752/68‑1 (MQ=255)                                              cgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCt                <  1:424465/68‑1 (MQ=255)                                                   ggCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGt           <  1:416085/68‑1 (MQ=255)                                                    gCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTg          <  1:1064443/68‑1 (MQ=255)                                                      gTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGcc        >  1:416954/1‑68 (MQ=255)                                                         taCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACt     >  2:955405/1‑68 (MQ=255)                                                         taCCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACt     <  1:1303913/68‑1 (MQ=255)                                                          aCCGCTGGGACTCCGGCGGAATCCGGAACGTCCCTGCCGGTGTAGTGACAGCCGCTGCCGTGCCACTg    >  2:1328341/1‑68 (MQ=17)                                                          aCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTg    >  2:1086008/1‑68 (MQ=255)                                                            cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGt  <  2:647444/68‑1 (MQ=255)                                                            cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGt  <  2:1170385/68‑1 (MQ=255)                                                            cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGt  <  1:604751/68‑1 (MQ=255)                                                            cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGt  <  1:1278709/68‑1 (MQ=255)                                                               |                                                                 GTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGT  >  NC_000913/264036‑264162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑