Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,197,502 C→T G11R (GGA→AGA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,197,5020CT94.7% 55.5 / NA 19G11R (GGA→AGA) ymfDe14 prophage; putative SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (7/11);  minor base A (1/0);  total (8/11)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGT  >  NC_000913/1197437‑1197543
                                                                 |                                         
cTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                         >  2:381595/1‑68 (MQ=255)
  cGCAAAGATATCTAGATGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGc                                       <  1:1231861/68‑1 (MQ=255)
  cGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGc                                       <  2:75274/68‑1 (MQ=255)
  cGCAAAGATATCTAGAGGGCATCGTATGAGGCTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGc                                       <  1:709758/68‑1 (MQ=255)
    cAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCat                                     <  2:927274/68‑1 (MQ=255)
      aaGAAATCTAGAGGGCATCGTATGAGGAATAGCTACATTCTATGAGCGAATAATAAATCAGTGCaaat                                   >  2:101421/1‑68 (MQ=255)
             cTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGtt                            >  1:1152146/1‑68 (MQ=255)
                  gggCTTCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTTACTCTGTGCATATTATAGTTCAAAg                       <  2:386539/68‑1 (MQ=255)
                  gggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAg                       >  1:249406/1‑68 (MQ=255)
                   ggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGc                      <  1:611164/68‑1 (MQ=255)
                    gCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                         >  1:280710/1‑48 (MQ=255)
                    gCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                         <  2:280709/48‑1 (MQ=255)
                    gCATCGTATGAGGTATTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCa                     >  1:386541/1‑68 (MQ=255)
                          tATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTAcc               <  2:249405/68‑1 (MQ=255)
                               cgTTTTGCATCACTCTCTGAGCGAATATTAACTCTGTGCTTATAAAAGTTCAAATCAAGTACCATTCa          <  2:569455/67‑1 (MQ=255)
                                gTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCaa         >  1:786171/1‑68 (MQ=255)
                                gTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCaa         <  1:21489/68‑1 (MQ=255)
                                                    cGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGt  <  1:651535/55‑1 (MQ=255)
                                                    cGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGt  >  2:651533/1‑55 (MQ=255)
                                                                 |                                         
CTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGT  >  NC_000913/1197437‑1197543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑