Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,679,525	G→T	A1074D (GCT→GAT)	yphG ←	DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,679,525	0	G	T	100.0%	78.6 / NA	24	A1074D (GCT→GAT)	yphG	DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/9);  total (15/9)

TGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCG  >  NC_000913/2679468‑2679588                                                          |                                                                tGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAAt                                                       >  2:336734/1‑68 (MQ=255)     cgcgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGACGCTTTCGGACAATCCGGATTACTCGcc                                                   >  2:633034/1‑68 (MQ=255)      gcgGTTAAAAATAAAAGGCATCACGGTGGCGAATAATGCCGCTTTCGGCCAATCCTGATTAATCGCCg                                                  <  2:1036136/68‑1 (MQ=255)           taataataaAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAt                                             <  1:1175927/68‑1 (MQ=255)                aTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAgc                                        >  1:463626/1‑68 (MQ=255)                     aGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAg                                   >  1:1185971/1‑68 (MQ=255)                         aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                               <  2:301819/68‑1 (MQ=255)                         aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                               <  2:1068998/68‑1 (MQ=255)                         aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATAAATCGCCGTCAATTCAGCGCGAGCAGa                               <  1:14685/68‑1 (MQ=255)                           cACGGTGGTGAATAATGCCGCTTTCGGCCTATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                             <  1:131173/68‑1 (MQ=255)                           cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                             <  1:880356/68‑1 (MQ=255)                           cACGGTGGTGAATAATGCCGCTTCCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                             <  2:1215450/68‑1 (MQ=255)                             cGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCa                           >  1:451380/1‑68 (MQ=255)                              ggtggtGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCat                          >  1:1184640/1‑68 (MQ=255)                                 ggtgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAt                                             >  1:949394/1‑46 (MQ=255)                                 ggtgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAt                                             <  2:949393/46‑1 (MQ=255)                                 ggtgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATAtt                       >  1:361011/1‑68 (MQ=255)                                      ataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa                  >  1:778427/1‑68 (MQ=255)                                      ataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa                  >  2:1181336/1‑68 (MQ=255)                                        aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc                >  1:1248281/1‑68 (MQ=255)                                           gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTc             >  2:316709/1‑68 (MQ=255)                                           gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTc             >  1:261350/1‑68 (MQ=255)                                                tttCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGa        >  2:730670/1‑68 (MQ=255)                                                      ccAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCcg  >  1:719078/1‑68 (MQ=255)                                                          |                                                                TGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCG  >  NC_000913/2679468‑2679588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑