Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,413,571	T→C	intergenic (‑106/‑293)	acrS ← / → acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,413,571	0	T	C	100.0%	39.1 / NA	12	intergenic (‑106/‑293)	acrS/acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/6);  total (6/6)

AAAATAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAAT  >  NC_000913/3413508‑3413629                                                                |                                                           aaaaTAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATaa                                                        >  1:592836/1‑68 (MQ=255)             gTTATTCTATCACCTAATGTTTATTTATACGAGAGGCTATTATTGAGTTGCCATATATCGTtaaataa                                            <  2:628254/68‑1 (MQ=255)                   cTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAataata                                      >  1:957820/1‑68 (MQ=255)                   cTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAataata                                      >  1:957821/1‑68 (MQ=255)                    tATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAatat                                     <  1:131867/68‑1 (MQ=255)                      tCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAatatat                                   <  1:194142/68‑1 (MQ=255)                      tCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGCTAAATAAATAatatat                                   <  2:592834/68‑1 (MQ=255)                            aaTGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATattatt                             >  1:961639/1‑68 (MQ=255)                                        taCGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATAc                 >  1:703918/1‑68 (MQ=255)                                            agagGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTc             >  1:892257/1‑68 (MQ=255)                                                      atTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCaa   <  1:439147/68‑1 (MQ=255)                                                       ttGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAat  <  2:703916/68‑1 (MQ=255)                                                                |                                                           AAAATAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAAT  >  NC_000913/3413508‑3413629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑