Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,215	Δ1 bp	coding (446/531 nt)	mprA →	transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,215	0	A	.	100.0%	85.2 / NA	20	coding (446/531 nt)	mprA	transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (11/9);  total (11/9)

ACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGT  >  NC_000913/2811156‑2811276                                                            |                                                              accaccGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGa                                                      >  2:271638/1‑68 (MQ=255) accaccGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGa                                                      <  1:632776/68‑1 (MQ=255)                ctgcctgcATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCaa                                       <  1:112198/68‑1 (MQ=255)                 tgcctgcATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCaaa                                      >  1:104490/1‑68 (MQ=255)                   cctgcATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAAtt                                    >  1:912226/1‑68 (MQ=255)                   cccgcATCAACTCTGGCCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAAtt                                    <  2:15550/65‑1 (MQ=255)                         tCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCtctc                              >  2:931593/1‑68 (MQ=255)                         tCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCtctc                              >  2:930224/1‑68 (MQ=255)                             ctctGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGt                          >  2:1152996/1‑68 (MQ=255)                             ctctGGTCCGCGCGCAGCACAACAGAAAAAG‑CCAGCTCGAGCAAATCACCCGCACACCGCCCTCCCGt                          <  2:35803/68‑1 (MQ=255)                              tctGGTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGtc                         >  1:292329/1‑68 (MQ=255)                              actGCTCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCACTCCCGtc                         >  1:229506/2‑68 (MQ=255)                                   tCCGCGCTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGAc                    >  1:894101/1‑68 (MQ=255)                                        gcTCAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGAt               <  1:285537/68‑1 (MQ=255)                                           cAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATgta            <  1:692720/68‑3 (MQ=255)                                           cAGCACAACAGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGa            <  2:894098/68‑1 (MQ=255)                                                  acaGAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGAc     >  1:440556/1‑68 (MQ=255)                                                    aGAAAAAG‑TCAGCTCGAGCCAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACgg   >  1:496323/1‑68 (MQ=255)                                                     gAAAAAG‑TCAGCTCGAGCTAAGCACCCTCAAATTGCTCTCCCGTCTCGACCTGATTGAACATGACTgt  <  1:180424/68‑1 (MQ=255)                                                     gAAAAAG‑TCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGgt  <  2:512223/68‑1 (MQ=255)                                                            |                                                              ACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGT  >  NC_000913/2811156‑2811276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑