| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 34,508 | 0 | G | C | 25.7% | 85.2 / 13.9 | 36 | G70A (GGA→GCA) | caiF | cai operon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (22/4); new base C (0/9); total (22/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTGCGCA > NC_000913/34441‑34573 | atTCTGTCGGAAGTCGCAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 2:36418/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAGGCTGCGAAGTTAAGATGATCACTAATAAGCTGGAAGGGCgggg > 2:1141312/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 1:237459/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 1:940383/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 1:728510/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 2:668421/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGATAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 2:239202/1‑68 (MQ=255) tCTGTCGGAAGTCACAGATATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGAt > 2:896564/1‑68 (MQ=255) tCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGAt > 1:948193/1‑68 (MQ=255) tCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGAt > 2:91218/1‑68 (MQ=255) ggATGTCACTGAAATAAGCTGCGAAGTTAATATTATCCCTAATAAGCTGGAAGGGCGGGCATGCCatt < 1:668423/68‑3 (MQ=255) gTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCa > 1:555107/1‑68 (MQ=255) ctaTAAGCTGCGAAGTTATGATGATCCCTACTAAGCTGGAAGGGCGGGCACGCCAGTGCCAGCGACTg < 2:483201/66‑1 (MQ=255) aaaTATGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGCATGCCAGTGTCAGCGACTg < 2:529646/68‑1 (MQ=255) aaaTAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTg > 2:672344/1‑68 (MQ=255) aaTAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTgg > 2:500626/1‑68 (MQ=255) gcagCGAAGTGAAGATGGTCCATGAAAAGCTGAAAGGGCGGGGATGCCTGAGTCAGCGACAGGTTaaa > 2:1138929/4‑68 (MQ=255) gCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTaaa > 1:54129/1‑68 (MQ=255) gCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCAAGTGTCAGCGACTGGTTaag > 2:115131/1‑67 (MQ=255) tGCGATGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGCATGCCAGTGTCAGCGACTGGTTAAAGt < 2:606561/68‑1 (MQ=255) ttAAGTTTATCCCTAATAAGCTGGAAGGGCGGGCATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGat < 2:920754/68‑1 (MQ=255) gatgatCCCTAATAAGCTGGAAGGGAGGGGATGCAAGTGTCAGAGACTGGTTTAAGTGGTCGatatag > 1:1073844/1‑66 (MQ=255) tgatCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGAt > 2:120667/1‑68 (MQ=255) tgatCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGAt > 2:744262/1‑68 (MQ=255) tgatCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGAt > 1:437226/1‑68 (MQ=255) cccTAATAAGCTGGAAGGGCGGGCATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGc < 1:1078205/68‑1 (MQ=255) atatGCTGGAAGGGCGTTCATGCCAGTGTCAGCTACTGGTTAAAGTGGGCGATATCGATGAGCATAtt < 1:304417/68‑1 (MQ=255) ataaGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAAtt > 1:61661/1‑68 (MQ=255) taaGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTgg < 1:909230/38‑1 (MQ=255) taaGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTgg < 1:751615/38‑1 (MQ=255) taaGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTgg > 2:909227/1‑38 (MQ=255) taaGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTgg > 2:751613/1‑38 (MQ=255) taaGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATTTCGATGAGCAAAttt > 1:77088/1‑68 (MQ=255) aaGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAGGTGGTCGATATCGATGAGCAAAtttg > 2:310258/1‑67 (MQ=255) aaGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTa > 1:570838/1‑68 (MQ=255) gCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTAcg > 2:157394/1‑68 (MQ=255) gCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTAcg > 2:2891/1‑68 (MQ=255) caaGGGCGGGCATGCCAGTGTCAGCGACTGGGTAAAGTGGCCGATATCGACGAGCAAATCTAcgcgcg < 2:815746/67‑1 (MQ=255) aaGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAGTTTCCgcgcgc > 1:805156/1‑68 (MQ=255) gCGGGCATGCCAGTGGCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTgc < 2:253515/68‑1 (MQ=255) cGGGTATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTgcg < 2:863527/68‑1 (MQ=255) cGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATTTCGATGAGCAAATTTACGCGCGCCTgcg < 2:77088/68‑1 (MQ=255) cGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTgcg < 2:364409/68‑1 (MQ=255) gggATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTGCGCa < 2:61661/68‑1 (MQ=255) | ATTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTGCGCA > NC_000913/34441‑34573 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |