| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,135,975 | 0 | G | C | 39.3% | 27.9 / 25.3 | 30 | A138P (GCC→CCC) | flgH | flagellar protein of basal‑body outer‑membrane L ring |
| Reads supporting (aligned to +/- strand): ref base G (12/5); new base C (0/11); total (12/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.53e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACG > NC_000913/1135912‑1136038 | tttGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGTCCaa < 2:113957/68‑1 (MQ=255) aaCGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAGCGGAAAGGGCGGGGCCAATGCCAg > 2:420090/1‑68 (MQ=255) aaCGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGccgg > 1:847404/1‑66 (MQ=255) aaCGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAg > 2:3614/1‑68 (MQ=255) aaCGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAg > 2:328009/1‑68 (MQ=255) aaCGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAg > 2:180700/1‑68 (MQ=255) aCGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGCCCAATGCCAGc < 1:508010/68‑1 (MQ=255) gCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGCCCAATGCCAGCaa < 2:823782/68‑1 (MQ=255) cTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAAt > 2:599509/1‑68 (MQ=255) cGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTa < 2:614674/68‑1 (MQ=255) aaGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGCCCAATGCCAGCAATACCTTTAGCGGCACg < 1:1145875/68‑1 (MQ=255) aaGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGCCCAATGCCAGCAATACCTTTAGCGGCACg < 2:648884/68‑1 (MQ=255) tAACACGTTCAACGGAAAGGGCGGGGCCAATGCCTGCAATACCTTTAGCGGCACGTtgactgtgacgg < 1:367572/68‑1 (MQ=255) acacGTTCAACGGAAAGGGCGGGCCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGtt < 2:1134175/68‑1 (MQ=255) cacTTTCAACGGAAAGGGCGGGTCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTg < 2:852034/68‑1 (MQ=255) cacGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAAAACCTTTAGCGGCACGTTGACGGTGACGGTTg > 2:564410/1‑68 (MQ=255) cGTTCAACGGAAAGGGCGGGCCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGAc < 2:252107/68‑1 (MQ=255) cGTTCAACGGAAAGGGCGGGCCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGAc < 1:642517/68‑1 (MQ=255) cGTTCAACGGAAAGGGCGGGCCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGAc < 1:628109/68‑1 (MQ=255) cGTGCAACGGAAAGGGCGGGCCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGAc < 2:524323/68‑1 (MQ=255) cAACGGAATGGGCGGGCCCAATGCCAGCAACACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAgg < 2:1113956/68‑1 (MQ=255) aaCGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGCCGGTTGACCAGGt > 2:573006/1‑68 (MQ=255) aaCGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGt < 1:3614/68‑1 (MQ=255) ggAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGCCGGTTGACCAGGTACt > 2:767657/1‑68 (MQ=255) ggAAAGGGCGGGGCCAATGCCAGCAAAACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACt > 2:840931/1‑68 (MQ=255) aaaGGGCGGGCCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTgg < 2:83144/68‑1 (MQ=255) acgggCGGGGCCAATGCCAGCAATACCTTAAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGt < 1:1028178/66‑1 (MQ=255) gggCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTg > 1:938872/1‑53 (MQ=255) gggCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTg < 2:938869/53‑1 (MQ=255) cGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGCCGGTTGACCAGGTACTGGTCAACg > 1:2496/1‑68 (MQ=255) | TTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACG > NC_000913/1135912‑1136038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |