| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,308,749 | 0 | G | C | 38.1% | 29.5 / 13.6 | 21 | intergenic (‑104/‑39) | clsA/yciY | cardiolipin synthase 1/uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (13/0); new base C (0/8); total (13/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAAC > NC_000913/1308688‑1308804 | cATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATaa > 1:486681/1‑68 (MQ=255) atGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATgg > 2:637590/1‑67 (MQ=255) aTTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCAt > 1:220667/1‑68 (MQ=255) aTTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCAt > 2:66619/1‑68 (MQ=255) caccTGTCAAATAAACCCGCTGGAAAAGCCCTGGTCTGCGGCGGGGGTGGGCCCACAATGACCATTCt < 2:486681/65‑1 (MQ=255) cATCTGCCAAATAAAACCGCTTGAAACGCACTGGTCTGCGGCGGGGGTGGGCCCACAATGACCATTCt < 2:222648/68‑1 (MQ=255) tCTGTCAAATAAAACCGCGTTTAACTCACTGGGCCGCGTCGGGGGTGGGCCCACAATGACCATTCTGt < 2:1131314/68‑1 (MQ=255) tCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGt > 1:323889/1‑68 (MQ=255) cTGGCAATTATATCCGCGGTAACCGCCCTGGTCTGCGGCGGGGGTGGGCCCATAATGACCATTCTGtt < 2:1140598/68‑1 (MQ=255) aTAAAATCGCGTTAAATGCACTGGTCAGCGTCGGGGGTGGGCGTATAATGACCATTCTGTTATTGCAt < 1:340505/68‑1 (MQ=255) aTAAAAACGCGTGAAAAGCACTGGTCAGCGTCGGGGGTGGGCGTATAATGACCATTCTGTTATTGCAt < 1:88770/68‑1 (MQ=255) aaaaCGCGGGAAAAGCACTGGGCTGCGGCGGGGGGGGCCCTATAATGACCATTCTGTTATTGCATaga < 1:746881/68‑1 (MQ=255) cgcgGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGagtag > 1:379100/1‑68 (MQ=255) cgcgGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGagtag > 2:1112790/1‑68 (MQ=255) aGCGCTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGa > 2:31348/1‑68 (MQ=255) tCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGt > 1:650245/1‑68 (MQ=255) tCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGt > 1:75008/1‑68 (MQ=255) gCGTCGGGGGTGGGCGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGa < 1:97397/68‑1 (MQ=255) gTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAAc > 2:522094/1‑68 (MQ=255) gTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAAc > 2:713617/1‑68 (MQ=255) gTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCAAAGAGTAGTTAACATGAAGCGGAGTAGAAc > 1:167062/1‑68 (MQ=255) ggtATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGt < 1:970529/51‑1 (MQ=255) ggtATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGt > 2:970525/1‑51 (MQ=255) | CATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAAC > NC_000913/1308688‑1308804 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |