| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,770,231 | 0 | C | G | 44.4% | 15.1 / 14.5 | 18 | intergenic (+85/+12) | yibU/yibH | pseudogene, H repeat‑associated protein/putative membrane fusion protein (MFP) component of efflux pump |
| Reads supporting (aligned to +/- strand): ref base C (0/10); new base G (8/0); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTAATGAAGTAATATGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATCT‑CCCCCCTCCCCCTCAATGATCCAAATAAAGATAATGCATCCAGCTGGTCAT > NC_000913/3770172‑3770281 | gCTAATGAAGTAATATGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCGATAATgt‑ggcccctcc > 2:437198/1‑68 (MQ=255) cTAATGAAGTAATATGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATct‑cccccctccc < 2:336785/68‑1 (MQ=255) aaTGAAGTAATATGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATct‑cccccctccccc < 1:844326/68‑1 (MQ=255) tGAAGTAATATGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATct‑gagccctaccccgc > 2:319497/1‑66 (MQ=255) gAAGTAATATGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATCT‑CCCCCCTCCCCCTCa < 2:514601/68‑1 (MQ=255) atGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAGTCT‑GAACCCTCCCCCTCAATGATCCa > 1:504431/1‑68 (MQ=255) tcAAAAATAATTAGTCCTTACAATGAGCAAAGTTCCATAATCT‑CCCCCCTCCCCCTCAATGATCCaaa < 2:808816/67‑1 (MQ=255) gCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCGATAGTGT‑GGGACCACACCCTCAATGATCaaaa > 2:582276/1‑68 (MQ=255) gCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATGT‑GGCCCCTCCCCCACAAAGATACaaa > 2:440907/1‑68 (MQ=255) gCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATCT‑CCCCCCTCCCCCTCAATGATCCaaa < 2:964512/68‑1 (MQ=255) gCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATCT‑CCCCCCTCCCCCTCAATGATCCaaa < 2:776581/68‑1 (MQ=255) cAAAAATAATTAGTCCTTACAATGAGCAAAGTTCCATAATCTCCCCCCCTCCCCCTCAATGATCCaaa < 1:232074/68‑1 (MQ=255) taatTAGTCCTTACAAGGAGCAAAGTTCCATAATCT‑CCCCCCTCCCCCTCAATGATCCAAATAAAGAt < 1:170892/68‑1 (MQ=255) ttAGTCCTTACAAGGAGCAAAGTTCCATAATCG‑GCGCCCGCCCCCACAATGATACAAAAAAAGATAAt > 1:617979/1‑68 (MQ=255) aGTCCTTACAAGGAGCAAAGTTCCATAATGT‑GGACCCGCCCCCACAATGATCCAAATAAAGaaaaaga > 2:1019657/1‑65 (MQ=255) aGTCCTTACAAGGAGCAAAGTTCCATAATCT‑CCCCCCTCCCCCTCAATGATCCAAATAAAGATAATGc < 2:1107128/68‑1 (MQ=255) aaGGAGCAAAGTTCCATAATCT‑CCCCCCTCCCCCTCAATGATCCAAATAAAGATAATGCATCCAGCTg < 1:709733/68‑1 (MQ=255) ggAGCAAAGTTGGATAATGT‑GGGCCCTCCCCCTCAATGATCCAAATAAAGATAATGAATCCAGCTGGt > 1:962491/1‑68 (MQ=255) ggAGCAAAGTTCGATAATGT‑GAACCCACCCCCACAATGATCCAAAAAAAGAAAATGAAACCAGCTggg > 2:137349/1‑67 (MQ=255) ggAGCAAAGTTCCATAATGT‑GGGCACGCCCCCACAAAGATACAAATAAAGATAATGAAAACAGCAGGt > 1:63571/1‑68 (MQ=255) gCAAAGTTCCATAATCT‑CCCCCCTCCCCCTCAATGATCCAAATAAAGATAATGCATCCAGCTGGTCAt < 1:437198/68‑1 (MQ=255) gCAAAGTTCCATAATCT‑CCCCCCTCCCCCTCAATGATCCAAATAAAGATAATGCATCCAGCTGGTCAt < 1:173344/68‑1 (MQ=255) | GCTAATGAAGTAATATGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATCT‑CCCCCCTCCCCCTCAATGATCCAAATAAAGATAATGCATCCAGCTGGTCAT > NC_000913/3770172‑3770281 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |