Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	264,098	0	G	T	100.0%	50.8 / NA	20	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/7);  total (13/7)

GTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTA  >  NC_000913/264036‑264149                                                               |                                                    gTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc                                                >  1:387637/1‑68 (MQ=255)      gCTCATCAGGCTGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCgg                                           >  1:166374/1‑68 (MQ=255)      gCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCgg                                           >  2:1036089/1‑68 (MQ=255)           tctggcaggTATAGGGCCCATTGCTGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGata                                      <  2:672909/65‑1 (MQ=38)           tcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGata                                      >  2:834671/1‑68 (MQ=255)            caggcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGatat                                     <  1:627870/68‑1 (MQ=255)                caggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCgg                                 >  2:63824/1‑68 (MQ=255)                caggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCgg                                 >  1:714524/1‑68 (MQ=255)                 aggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGa                                <  2:714522/68‑1 (MQ=255)                     ttaGGGCCGTTTGCGGTCCTGGCTGCGTTCGTCGTATACCGCTTGGACGCCGTCGATATCCGGTACGt                            <  2:333438/67‑1 (MQ=255)                     ataGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGt                            >  2:588322/1‑68 (MQ=255)                          gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                       >  1:1082933/1‑68 (MQ=255)                          gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                       >  2:298511/1‑68 (MQ=255)                          gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                       >  1:662804/1‑68 (MQ=255)                               ttGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt                  <  1:1073836/68‑1 (MQ=255)                                     tCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATg            >  1:864487/1‑68 (MQ=255)                                          gcggcgTTCTGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGa       <  1:406250/68‑1 (MQ=255)                                               gTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTa  >  1:376281/1‑68 (MQ=255)                                                           ccGCTATGACTCCGGCGTTATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTa  <  1:455795/56‑1 (MQ=17)                                                           ccGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTa  >  2:455795/1‑56 (MQ=255)                                                               |                                                    GTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTA  >  NC_000913/264036‑264149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑