| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,413,571 | T→C | intergenic (‑106/‑293) | acrS ← / → acrE | acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,413,571 | 0 | T | C | 96.0% | 84.4 / NA | 25 | intergenic (‑106/‑293) | acrS/acrE | acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (10/14); minor base A (1/0); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAATAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAATATA > NC_000913/3413508‑3413632 | aaaaaaGCGAAGGGTAATCTATCAACTAATGTGTATATATAAGAGAGGCTAATATTCAAATGCCATaa > 2:182490/1‑68 (MQ=255) aTAGCGAAGGTTAATCTATCACCTAATGCGTATTTATACGAGAGGCTAATATTGAGTCGCCATAAATc < 2:1138763/68‑1 (MQ=255) gCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGtt < 1:663118/68‑1 (MQ=255) cGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTta < 2:274782/68‑1 (MQ=255) aGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaat < 2:933849/68‑1 (MQ=255) tttAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaatta < 1:91053/67‑3 (MQ=255) gTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTATTATTGAGTTGCCATAAATCGTtaaataa < 2:700510/68‑1 (MQ=255) cTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaataa < 1:327561/62‑1 (MQ=255) cTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaataa > 2:327561/1‑62 (MQ=255) tCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAatatat < 2:198684/68‑1 (MQ=255) aCCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAatatatat > 2:100391/1‑68 (MQ=255) ccTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATAtatt < 2:615615/68‑1 (MQ=255) aTGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTAttt < 2:1115040/68‑1 (MQ=255) gtgtATTTATACGAGAGGCTAATATTGAGTTGCAATAAATCGATAAATAAATAATATATATTAATTAc > 2:351840/1‑68 (MQ=255) gtATTTATACGAGAGGCTAAAATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCt < 2:125299/68‑1 (MQ=255) aTTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAaataaata > 2:423749/1‑49 (MQ=255) aTTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAaataaata < 1:423749/49‑1 (MQ=255) agagGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTc > 1:778116/1‑68 (MQ=255) atatTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATc < 1:215698/68‑1 (MQ=255) atTGAGATGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCaa > 1:38522/1‑68 (MQ=255) ttGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAat > 1:272562/1‑68 (MQ=255) aGTTGCCATAAGTCGTTAAATAAATAATATATATTATATACCTAAGATACATTCACTACATCAatata > 2:1126126/1‑68 (MQ=255) aGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAatata > 2:1126127/1‑68 (MQ=255) aGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAatat > 2:836081/1‑67 (MQ=255) aGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAatat < 1:836084/67‑1 (MQ=255) | AAAATAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAATATA > NC_000913/3413508‑3413632 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |