| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,046,078 | 0 | G | A | 35.3% | 11.7 / 13.3 | 17 | intergenic (‑177/+90) | ygfF/gcvP | putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex |
| Reads supporting (aligned to +/- strand): ref base G (3/8); new base A (6/0); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.05e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTT > NC_000913/3046016‑3046145 | ggTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCTCCCCCAAACCCACTAACCAAAAGGGCg > 1:451369/1‑68 (MQ=21) caggTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg < 2:320448/66‑1 (MQ=255) gcCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACt < 1:42436/68‑1 (MQ=255) cAGTGCCTGCCTGTTCCCCTCACCCTAACCCTCTCCCCAATGGGGCGAGGGGACTGTCTGAGCACttt < 1:332379/68‑1 (MQ=18) cAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACttt < 1:306411/68‑1 (MQ=255) tGCCGGCCCGTCCCCCTCACCCTAACCCTCCCCCCAATGGGGCGAGGGGACTGTCTTAGCACTTTTGt < 2:32110/68‑1 (MQ=18) gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGGCTGAGCACTTTTGTACTTTGTCa < 2:155614/68‑1 (MQ=255) cccTCTCCCTGAAAGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGgcg > 1:322469/1‑68 (MQ=37) ccTCTCCCTGAAAGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGgcgc > 1:111256/1‑68 (MQ=38) ccTCTCCCTGAAAGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGgcgc > 2:290561/1‑68 (MQ=38) ccTCTCCCTGAAAGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGgcgc > 2:222436/1‑68 (MQ=38) ccTCTCCCTGAAAGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGgcgc > 2:232010/1‑68 (MQ=38) ccTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGgcgc > 1:421765/1‑68 (MQ=255) ccTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGgcgc > 2:282645/1‑68 (MQ=255) tctcCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCg > 1:672666/1‑68 (MQ=255) aaaGGGGCGATGGGACTGTCTGAGCACTCTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGcc < 2:212953/68‑1 (MQ=255) aaaGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGcc < 2:387718/68‑1 (MQ=255) ggggCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCttt > 2:494783/1‑68 (MQ=255) ggggCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCttt < 2:394100/68‑1 (MQ=255) ggggCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCttt > 1:362019/1‑68 (MQ=255) ggggCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCttt > 1:127951/1‑68 (MQ=255) | GGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTT > NC_000913/3046016‑3046145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |