Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	264,098	0	G	T	100.0%	47.0 / NA	18	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/6);  total (12/6)

TCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGG  >  NC_000913/264046‑264161                                                     |                                                                tcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGata                                                  <  2:513530/68‑1 (MQ=255) tcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGGTCGGCGTATACAGCTAGGACTCCGGCGaag                                                  >  2:471314/1‑66 (MQ=255) tcaGGCAGGTATAGGGCACATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGata                                                  >  1:633562/1‑68 (MQ=255)  caggcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGatat                                                 <  2:346882/68‑1 (MQ=255)           ataGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGt                                        >  2:529069/1‑68 (MQ=255)               ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCt                                    >  2:669537/1‑68 (MQ=255)               ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCt                                    >  1:98580/1‑68 (MQ=255)               ggCCCATTGCGGTCCAGGCGGAGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGCCCGTCCCt                                    >  2:56570/1‑68 (MQ=255)                gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                   >  2:201223/1‑68 (MQ=255)                    aTTGCTGTCCTTGCGGCGTTCGTCTTATACCGCTAGGACTCAGTCGATATCCGGAACGTCCCTGCCgg                               <  1:228995/68‑1 (MQ=21)                      tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                             <  2:506165/68‑1 (MQ=255)                       gCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCACTGCCGgtgt                            >  2:9113/1‑68 (MQ=21)                            ccTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGa                       >  2:412597/1‑68 (MQ=255)                                 cggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGAc                  >  2:493955/1‑68 (MQ=255)                                    ctttCTGCGTATACCGCTAGGACTCCGGCGATTTCCGGTACGTCCCTTCCGGTGTAATGACAGACGCt               <  2:49987/66‑1 (MQ=18)                                                 ccGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTgg  >  2:443461/1‑68 (MQ=255)                                                 ccGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTgg  <  1:324043/68‑1 (MQ=255)                                                 ccGCTAGGACTCCGGCGATATCCGGAACGTCCCAGCCGGTGTAATGACAGACGCAACAGTGCCACAgg  >  1:671991/1‑68 (MQ=17)                                                     |                                                                TCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGG  >  NC_000913/264046‑264161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑