Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,679,525	G→T	A1074D (GCT→GAT)	yphG ←	DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,679,525	0	G	T	100.0%	72.7 / NA	22	A1074D (GCT→GAT)	yphG	DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/9);  total (13/9)

GGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAG  >  NC_000913/2679460‑2679583                                                                  |                                                           gggTTTAGTGAACGCGGTTTAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATcc                                                          >  1:29566/1‑68 (MQ=255)   gTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCgg                                                        <  2:393177/68‑1 (MQ=255)   gTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGACGCTTTCGGCCAATCCgg                                                        >  1:467036/1‑68 (MQ=255)     ttAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGAt                                                      <  1:666482/68‑1 (MQ=255)        gTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTaa                                                   >  2:243487/1‑68 (MQ=255)         tGAACGCGGTTAAAAAGAAAAGGCATCACGGTGGTGAATAGTGCCGCTTTCGGCCGATCCGGATTAAt                                                  >  2:635907/1‑68 (MQ=255)                            aaGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGa                               >  1:434168/1‑68 (MQ=255)                                 aTCACGGTTGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                          <  2:434166/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                          <  1:368963/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                          <  1:48046/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                          <  2:368266/68‑1 (MQ=255)                                   cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                        >  1:94909/1‑68 (MQ=255)                                      ggtggtGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTTAGCGCGAGCAGACTCat                     <  1:96037/68‑1 (MQ=255)                                      ggtggtGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCat                     >  1:154094/1‑68 (MQ=255)                                         ggtgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATAtt                  >  2:348540/1‑68 (MQ=255)                                                aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc           >  2:558390/1‑68 (MQ=255)                                                aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc           >  2:142885/1‑68 (MQ=255)                                                aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc           >  1:676827/1‑68 (MQ=255)                                                    ccGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTcc       <  2:397839/68‑1 (MQ=255)                                                     cGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTccc      <  2:65915/68‑1 (MQ=255)                                                        tttCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCgc   >  1:330596/1‑67 (MQ=255)                                                         ttCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGAGAGCAGACTCATATTGCGCAACCTCCCCGAg  >  2:390178/1‑68 (MQ=255)                                                                  |                                                           GGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAG  >  NC_000913/2679460‑2679583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑