Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,095	A→C	D109A (GAT→GCT)	mprA →	transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,095	0	A	C	100.0%	32.1 / NA	10	D109A (GAT→GCT)	mprA	transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (3/7);  total (3/7)

GCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGG  >  NC_000913/2811031‑2811151                                                                 |                                                         gcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGCTcg                                                       >  2:213163/1‑68 (MQ=255)     cgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGCTcgccgc                                                   <  2:393901/68‑1 (MQ=255)          aTTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGCTCGCCGctgcct                                              <  1:33551/68‑1 (MQ=255)                    aaCTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGCTCGCCGCTGCCTGCATCTGCaa                                    <  1:495417/68‑1 (MQ=255)                    aaCTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGCTCGCCGCTGCCTGCATCTGCaa                                    >  2:497733/1‑68 (MQ=255)                           aaaaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGCTCGCCGCTGCCTGCATCTGCAATTAACgg                             <  1:482354/68‑1 (MQ=255)                           aaaaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGCTCGCCGCTGCCTGCATCTGCAATTAACgg                             <  2:199179/68‑1 (MQ=255)                                  ggTTGGATCGAACGTCGTGAAAGCGATAACGCTCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAgg                      <  1:1425/68‑1 (MQ=255)                                          cGAACGTCGTGGGAGCGATAACGCTCGCCGCTGCCTGCGTCTGCGATTAGCGGAAGAGGGTGGCGAGt              >  2:242475/1‑68 (MQ=255)                                                      aaGCGATAACGCTCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAgg  <  1:254070/68‑1 (MQ=255)                                                                 |                                                         GCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGG  >  NC_000913/2811031‑2811151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑