Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,413,571	T→C	intergenic (‑106/‑293)	acrS ← / → acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,413,571	0	T	C	100.0%	49.0 / NA	14	intergenic (‑106/‑293)	acrS/acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/6);  total (8/6)

TAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAATA  >  NC_000913/3413512‑3413630                                                            |                                                            tAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCg                                                     >  2:247740/1‑68 (MQ=255)        ggTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaata                                              >  1:41572/1‑68 (MQ=255)               cTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAataata                                       >  1:61366/1‑68 (MQ=255)                           gtgtATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTAc                           <  1:192633/68‑1 (MQ=255)                            tgtATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTAca                          >  1:633546/1‑67 (MQ=255)                            tgtATTTATACGAGAGGCTAATATTGAGTCTCCATAAATCGTTAAATAAATAATATATATAATTTAcc                          <  2:648723/68‑1 (MQ=255)                               aTTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTat                       >  2:153199/1‑67 (MQ=255)                                 ttATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGa                     <  1:496818/68‑1 (MQ=255)                                 ttATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGa                     <  2:652932/68‑1 (MQ=255)                                         gagGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCa             <  1:486168/68‑1 (MQ=255)                                              tAATATAGAGGTGCCATAATTCGTTAAATAAATATTATAATTTATTTACCTAAGATACATTCACTTCa        <  1:410629/68‑1 (MQ=255)                                                  atTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCaa    >  1:531780/1‑68 (MQ=255)                                                  atTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCaa    >  1:675939/1‑68 (MQ=255)                                                    tGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAata  >  1:287645/1‑68 (MQ=255)                                                            |                                                            TAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAATA  >  NC_000913/3413512‑3413630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑