Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,679,525	G→T	A1074D (GCT→GAT)	yphG ←	DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,679,525	0	G	T	100.0%	89.0 / NA	27	A1074D (GCT→GAT)	yphG	DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/13);  total (14/13)

GGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCG  >  NC_000913/2679460‑2679588                                                                  |                                                                gggTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATcc                                                               >  2:633731/1‑68 (MQ=255)   gTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCgg                                                             >  2:719150/1‑68 (MQ=255)         tGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAAt                                                       >  1:807744/1‑68 (MQ=255)         tGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAAt                                                       >  2:139067/1‑68 (MQ=255)         tGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAAt                                                       >  2:450681/1‑68 (MQ=255)          gAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGAGTAATc                                                      >  1:839424/1‑68 (MQ=255)             cgcgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGcc                                                   >  2:771957/1‑68 (MQ=255)              gcgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCg                                                  <  1:633730/68‑1 (MQ=255)                             aGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAg                                   <  2:810518/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATTCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                               <  2:495509/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCTGATTAATCGCCGCCACTTCAGCGCGAGCAGa                               <  2:706538/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                               <  2:209541/68‑1 (MQ=255)                                 aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGGCAATTCAGCGCGAGCAGa                               <  2:81618/68‑1 (MQ=255)                                   cACGGTGGTGAATAATGCCGCTTTCGTCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                             <  2:563629/68‑1 (MQ=255)                                   cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCTGATTATTCGCCGTCAATTCAGCGCGAGCAGACt                             <  2:234913/68‑1 (MQ=255)                                   cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                             <  2:471349/68‑1 (MQ=255)                                   cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                             <  1:406010/68‑1 (MQ=255)                                      ggtggtGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCat                          >  2:173184/1‑68 (MQ=255)                                      ggtggtGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCat                          <  1:478165/68‑1 (MQ=255)                                                aatGCCGCTTTCGGCCAATCCTGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                             <  2:480243/55‑1 (MQ=39)                                                aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                             >  1:480242/1‑55 (MQ=255)                                                aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc                >  1:779977/1‑68 (MQ=255)                                                aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc                >  2:768664/1‑68 (MQ=255)                                                aatGCCGCTTTCGGCCAATCAGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc                >  2:64704/1‑68 (MQ=255)                                                     cGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                             <  2:507215/50‑1 (MQ=255)                                                     cGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                             >  1:507214/1‑50 (MQ=255)                                                              ccAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCcg  >  1:744430/1‑68 (MQ=255)                                                                  |                                                                GGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCG  >  NC_000913/2679460‑2679588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑