| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 34,508 | 0 | G | C | 35.7% | 63.6 / 32.7 | 42 | G70A (GGA→GCA) | caiF | cai operon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (21/6); new base C (0/15); total (21/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.10e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTGCGCA > NC_000913/34441‑34573 | atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 2:970818/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 1:1137458/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 2:137395/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 2:42831/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 1:986056/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 1:863391/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCgggg > 1:8174/1‑68 (MQ=255)atTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCACTAATAAGCTGGAAGGGCgggg > 1:639843/1‑68 (MQ=255) tCTGTCGGAAGTCACAGATATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGAt > 1:257298/1‑68 (MQ=255) cTGGCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGCATg < 2:807277/68‑1 (MQ=255) gTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTcg > 2:875417/1‑67 (MQ=255) gTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCa > 2:155584/1‑68 (MQ=255) gTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCa > 2:850064/1‑68 (MQ=255) acaTAAATAAGCTGCGATGTTATGATGACCCCTAATATGCTGGATGGCCGGGCATGCCAGTGTCAGCg < 2:395300/68‑1 (MQ=255) aGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGCATGCCAGTGTCAGCGAc < 2:35285/68‑1 (MQ=255) aaaTAAGCTGCGAAGTTTAGATGATCCCTAATAAGCTGGATGGGCGGGCATGCCAGTGTCAGCGACTg < 2:68011/68‑1 (MQ=255) aaaTAAGCTGCGAAGTTAAGATGATCCCTAATAATCTTGAAGGGCGGGCATGCCAGTGTCAGCGACTg < 1:39242/68‑1 (MQ=255) aaaTAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGTAAGGGCGGGCATGCCAGTGTCAGCGACTg < 1:714590/68‑1 (MQ=255) aaaTAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGCATGCCAGTGTCAGCGACTg < 1:1192272/68‑1 (MQ=255) aaGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTa > 1:183549/1‑68 (MQ=255) aGCTGCGAAGTTAAGATGATCCCTAATAAGCTGTAAGGGCGGGCATGCCAGTGTCAGCGACTGGTTaa < 1:879237/68‑1 (MQ=255) gCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTaaa > 2:648569/1‑68 (MQ=255) gCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTaaa > 2:1162799/1‑68 (MQ=255) gCTGAGAAGTTAAGATGATCCCTAATAAGCGGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTtaa > 2:763073/1‑68 (MQ=255) tGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGCATGCCAGTGTCAGCGACTGGTTAAAGt < 2:176360/68‑1 (MQ=255) ctaaGTTAAGACGATCCCTAATAAGCTGGACGGGCGGGCATGCCAGTGTCAGCGACTGGTTAAAGTgg < 2:437567/66‑1 (MQ=255) cGAAGTTAAGATGATCCCTAATATGCTGGAAGGGCGGGCATGCCAGTGTCAGCGACTGGTTAAAGTgg < 2:877559/68‑1 (MQ=255) cGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGCgg < 2:183549/68‑1 (MQ=255) cGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGCATGCCAGTGTCAGCGACTGGTTAAAGTgg < 1:657764/68‑1 (MQ=255) cGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGCATGCCAGTGTCAGCGACTGGTTAAAGTgg < 1:171054/68‑1 (MQ=255) ttAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGGCAGCGACTGGTTAAAGTGGTCGat < 2:1054669/68‑1 (MQ=255) gatgatCCCTATTAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCg > 1:567366/1‑68 (MQ=255) gatgatCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCg > 2:488978/1‑68 (MQ=255) tgatCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCGGCGACTGGTGAAAGTGGTCGATATCGAt > 2:1034055/1‑68 (MQ=255) tgatCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTTAAGTGGTCGATATCGAt > 1:1058608/1‑68 (MQ=255) tgatCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGAt > 2:606802/1‑68 (MQ=255) tgatCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGAt > 1:487041/1‑68 (MQ=255) tgatCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGAt > 2:725313/1‑68 (MQ=255) atCCCTAATAAGCTGGAAGGGCGGGCATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGa < 1:178564/68‑1 (MQ=255) ccTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCa > 2:1045912/1‑68 (MQ=255) aataaGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAAt < 2:921532/68‑1 (MQ=255) tacgCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTTGATAAAGTGGTCGAAATCGATGGGCAAAttt > 1:271388/4‑68 (MQ=255) taaGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGat < 2:1183783/52‑1 (MQ=255) taaGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGat > 1:1183785/1‑52 (MQ=255) aaGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTa > 1:1172284/1‑68 (MQ=255) gCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTAcg > 1:1137464/1‑68 (MQ=255) gacgggcgggcAGCCCAGTGTCAGCGACTGTGTAAAGTGGTCGATATCGATGAGCAAACTTAcgcgcg < 2:1137456/65‑1 (MQ=255) gAAGGGCGGGCATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGACGAGCAAATTTAcgcgcg < 2:676417/68‑1 (MQ=255) aaGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACgcgcgc > 2:713032/1‑68 (MQ=255) gCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTgc < 1:1045913/68‑1 (MQ=255) cGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTgcg < 2:863391/68‑1 (MQ=255) gggATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTGCGCa < 2:1137462/68‑1 (MQ=255) | ATTCTGTCGGAAGTCACAGAAATAAGCTGCGAAGTTAAGATGATCCCTAATAAGCTGGAAGGGCGGGGATGCCAGTGTCAGCGACTGGTTAAAGTGGTCGATATCGATGAGCAAATTTACGCGCGCCTGCGCA > NC_000913/34441‑34573 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |