| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,563,398 | 0 | G | C | 38.5% | 30.1 / 11.5 | 28 | S779C (TCC→TGC) | dosP | oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator |
| Reads supporting (aligned to +/- strand): ref base G (10/6); new base C (0/10); total (10/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.55e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGT > NC_000913/1563334‑1563462 | tCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGaaa > 2:372900/1‑68 (MQ=255) ctgCGGTTACACGCTGCTCATCCAGCCCGGAATTTCTTCGGCGGGTTGGTGGCGGTCAAAGTATTATc < 2:377875/66‑1 (MQ=255) cAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATc < 2:543269/68‑1 (MQ=255) cAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAAGAAATATc < 2:668277/68‑1 (MQ=255) ggcaaCACGCTGCTCATCCAGCCTGGAACTTCTTCGGCGGGTAGGGGGCGGCAAAAGAAATATCCCTg < 2:1177834/65‑1 (MQ=255) acGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAAc > 1:581689/1‑68 (MQ=255) acGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAAGAAATATCCCTGAATAAc < 1:216350/68‑1 (MQ=255) gctgctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAAcgc > 2:937518/1‑68 (MQ=255) gctgctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAAcgc > 2:879372/1‑68 (MQ=255) gctCATCCTGCCCGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAAGAAATATCCCTGAATAACGCGAc < 1:1104175/68‑1 (MQ=255) gctCATCCAGCCTGGAATTTCTTCGGCGTGTAGGGGGCGGCAAAAGAAATATCCCTGAATAACGCGAc < 2:703205/68‑1 (MQ=255) gctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGAc < 2:932511/68‑1 (MQ=255) gctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGAc < 2:173441/68‑1 (MQ=255) gctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAACAAATATCCCTGAATAACGCGAc < 1:1032728/68‑1 (MQ=255) gctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAAGAAATATCCCTGAATAACGCGAc < 2:818084/68‑1 (MQ=255) gctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAAGAAATATCCCTGAATAACGCGAc < 2:670657/68‑1 (MQ=255) gctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAAGAAATATCCCTGAATAACGCGAc < 2:546587/68‑1 (MQ=255) tCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAg > 2:1073721/1‑68 (MQ=255) cATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAAGAAATATCCCTGAATAACGCGACAGt < 2:570957/68‑1 (MQ=255) aTCCTGCCTGGAATTTCTTCTGCGTGTAGGGGTCGGGAAAAGAAATATCCCTGAATAACGCGACAGTg < 2:581689/68‑1 (MQ=255) cAGCCTGGAATTTCTTCGGCGGGTAGGGTGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGAt < 1:1105219/68‑1 (MQ=255) cAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGAt > 1:98528/1‑68 (MQ=255) ccTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCtt > 1:16152/1‑68 (MQ=255) cTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATACCTGAATAACGCGACAGTGGATCTTg > 2:525738/1‑68 (MQ=255) ttcGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCAtct > 2:913715/1‑68 (MQ=255) gggggCGGCAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGctct < 1:1038456/68‑1 (MQ=255) gggCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCttt > 1:7592/1‑68 (MQ=255) cGGTAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGt < 1:340668/68‑1 (MQ=255) | TCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGT > NC_000913/1563334‑1563462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |