| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,253,024 | 0 | C | G | 38.1% | 22.7 / 11.2 | 21 | G374G (GGG→GGC) | nupX | nucleoside permease |
| Reads supporting (aligned to +/- strand): ref base C (3/10); new base G (8/0); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGG > NC_000913/2252963‑2253076 | gcgcCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCccacc < 1:245574/68‑1 (MQ=255) ccGTATTCCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGTAAACGCCCccaccacca < 2:965704/68‑1 (MQ=37) ccGTAATCCTAGCTGGGAGATTTCCGTCTCTCGGTGTGGCGCAACCGCAGAAAACTCCCcttccacca < 2:605730/68‑1 (MQ=255) gTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCaccaccacc < 1:859021/68‑1 (MQ=255) aaaCCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAACCGCGCCCCCCACCAcccc > 1:957183/1‑68 (MQ=37) aGCTGTGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGa < 2:623748/68‑1 (MQ=255) aGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGa < 2:383957/68‑1 (MQ=255) aGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGa < 2:163293/68‑1 (MQ=255) gCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGGGCCCACCACCACCCCGATAGaa > 2:92383/1‑68 (MQ=255) gCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGGCCCCCCCCCCACACCGCTAgca > 1:799688/1‑66 (MQ=25) gcgcACGGTGTGGCGCAACCGCAGAAAACGGCCCCACCACCACCCCGATAGAACCAAAGTTAGCGaaa > 2:797536/1‑68 (MQ=255) cgcACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAAc < 2:1039632/68‑1 (MQ=255) gcggCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAAc < 2:432021/66‑1 (MQ=255) gcgcAACCGCAGAAAAGGGGCCCACCACCCCCCCGATAGAACCAAAGTTAGCGAAACCGCACAAcgcg > 2:972349/1‑68 (MQ=37) gcgcAACCGCAGAAAAGGGGCCCACCACCACCCCGATAGAACCAAAGTTAGCGGAACCGCACAAcgcg > 1:207070/1‑68 (MQ=37) gcACCCGCAGAAAACGGGCCCGCCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGaa > 1:77511/1‑68 (MQ=37) gcAACCGCAGAAACCGGCCCCCCCCCCCCCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGaa > 2:1017711/1‑68 (MQ=37) gcAACCGCAGAAAAGGGGCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACGAcgcgga > 1:887528/1‑66 (MQ=25) gcAACCGCAGAAAAGGGGCCCACCACCAACCCGATAGAACCAAAGTTAGCGAAACCGCACAAcgcgga > 1:432021/1‑66 (MQ=37) gcAACCGCAGAAAAAGGGCCCACCACCACCCCGATAGAAACAAAGTTAGCGAAACCGCACAACGCGaa > 1:373974/1‑68 (MQ=255) aaCCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAgg < 2:851788/68‑1 (MQ=255) | GCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGG > NC_000913/2252963‑2253076 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |