Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,178,227	T→G	V162G (GTT→GGT)	nusG →	transcription termination factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,178,227	0	T	G	100.0%	93.2 / NA	26	V162G (GTT→GGT)	nusG	transcription termination factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/11);  total (15/11)

GTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACC  >  NC_000913/4178162‑4178290                                                                  |                                                                gTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGgtt                                                               <  1:802756/68‑1 (MQ=255)   tCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCt                                                             >  2:1026939/1‑68 (MQ=255)     gCTTACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGATAGTGTCTGGTTCttt                                                           <  1:524170/68‑3 (MQ=255)           ttCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCgg                                                     <  1:678802/68‑1 (MQ=255)               aCGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGt                                                 >  1:554533/1‑68 (MQ=255)                 ggTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGTGc                                               >  2:979016/1‑68 (MQ=255)                 ggTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGTGc                                               >  2:883999/1‑68 (MQ=255)                 ggTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGTGc                                               >  2:1042965/1‑68 (MQ=255)                 ggTGTTGATGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGACTGGATCAATCATCGGTCGTGc                                               >  1:832148/1‑68 (MQ=255)                   tgttgtTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGTGCGa                                             <  2:1025763/68‑1 (MQ=255)                    gatgttgAAGAAGTGGAATACGAGAAATAACGTCTGAAAGAGTCTGGTTCTATATACGGTCGTGCGAc                                            >  2:563810/3‑68 (MQ=255)                     ttgttgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGTGCGAcc                                           <  2:865154/68‑1 (MQ=255)                     ttgttgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGTGCGAcc                                           <  2:1044981/68‑1 (MQ=255)                     ttgttgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGTGCGAcc                                           <  2:639382/68‑1 (MQ=255)                       gttgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGTGCGAcccc                                         >  2:218113/1‑68 (MQ=255)                        ttgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGTGCGACCCCg                                        <  1:432295/68‑1 (MQ=255)                         tgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGTGCGACCCCgg                                       >  2:643465/1‑68 (MQ=255)                            agaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGTGCGACCCCGGTag                                    >  2:541166/1‑68 (MQ=255)                             gaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGTGCGACCCCGGTaga                                   <  2:60918/68‑1 (MQ=255)                             gaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGTGCGACCCCGGTaga                                   <  1:883999/68‑1 (MQ=255)                             gaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGTGCGACCCCGGTaga                                   <  1:1042966/68‑1 (MQ=255)                              aagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGTGCGACCCCGGTagag                                  >  2:381577/1‑68 (MQ=255)                                    aTTACGAGAAATCTCGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGAc                            <  1:434141/68‑1 (MQ=255)                                    aTTACGAGAAATCTCGTCTGAAAGTGTCAGGTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGAc                            >  2:518564/1‑68 (MQ=255)                                               tctcGTCTGAAAGTGTCTGGTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGt                 >  2:993614/1‑68 (MQ=255)                                                          aGTGTCTGGTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCt      >  2:564498/1‑68 (MQ=255)                                                              tCTGGTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAAcc  >  2:495601/1‑68 (MQ=255)                                                                  |                                                                GTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACC  >  NC_000913/4178162‑4178290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑