Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	264,098	0	G	T	100.0%	59.5 / NA	22	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/12);  total (10/12)

GTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGG  >  NC_000913/264036‑264161                                                               |                                                                gTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc                                                            >  2:365342/1‑68 (MQ=255) gTGATGCTCATAAGGAAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc                                                            >  1:971578/1‑68 (MQ=38)        tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCg                                                     >  1:680311/1‑68 (MQ=255)           tcaGGCAGGTATAGGTCCCTTTGCGCTCCTGGCGGCGTTCTGCGTATACCTCTTGGACTCCGGCGata                                                  <  2:963575/68‑1 (MQ=255)            caggcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGatat                                                 <  2:23799/68‑1 (MQ=255)            caggcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGatat                                                 <  2:75148/68‑1 (MQ=255)                caggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCgg                                             >  1:659811/1‑68 (MQ=255)                caggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCgg                                             >  1:659810/1‑68 (MQ=255)                caggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCgg                                             <  1:239787/68‑1 (MQ=255)                     atatGGTCCATTGATGTCCTGTCGTCGTGCGGCGTATACCGCTTGGACTCCGGCGATATCCGGAACGt                                        <  2:327859/68‑1 (MQ=19)                     ataGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGt                                        <  2:1068042/68‑1 (MQ=255)                       aGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTcc                                      <  1:453195/68‑1 (MQ=255)                         ggCACATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAAAGTccat                                    >  2:284176/1‑66 (MQ=21)                          gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                   >  2:277504/1‑68 (MQ=255)                                tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                             >  2:201345/1‑68 (MQ=255)                                         ggcggcGTGCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAg                    <  2:748201/68‑1 (MQ=255)                                           cggcgTTCTGCGTATACCGCTAGGACTCCGGCGTTATCCGGAACGTCCCTGCCGGTGTAAtgactgac                  <  2:705003/68‑1 (MQ=18)                                           cggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGAc                  <  2:705002/68‑1 (MQ=255)                                            ggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACg                 >  1:976907/1‑68 (MQ=255)                                              cgTGCGGCGTTTACCGCTTTGACTCCTGCGATATCCGGAACGTCCCTTCCGGTGTAATGACAGACGCt               <  1:284176/68‑1 (MQ=14)                                                      gTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGcc       <  1:1064155/68‑1 (MQ=255)                                                           ccGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGCCGCTACCGTGCCACTgg  >  2:437059/1‑68 (MQ=255)                                                               |                                                                GTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGG  >  NC_000913/264036‑264161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑