Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,095	A→C	D109A (GAT→GCT)	mprA →	transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,095	0	A	C	100.0%	39.4 / NA	12	D109A (GAT→GCT)	mprA	transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (2/10);  total (2/10)

GACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTT  >  NC_000913/2811033‑2811153                                                               |                                                           gacgcgTATTGCCGATGAACAGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGCTcgcc                                                       <  1:1135056/68‑1 (MQ=255)           gCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGCTCGCCGCTGCCTGCa                                             <  2:546897/68‑1 (MQ=255)           gCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGCTCGCCGCTGCCTGCa                                             <  2:979989/68‑1 (MQ=255)           gCCGATGAACTGGAAAAACGCGGGTGGATCGAACGTCGTGAAAGCGATAACGCTCGCCGCTGCCTGCa                                             <  1:66342/68‑1 (MQ=255)                  aaCTGGAAAGACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGCTCGCCGCTGCCTGCATCTGCag                                      >  2:592234/1‑67 (MQ=255)                          aaaCGCGGTTGGATCGAACGGCGTGAAAGCGATAACGCTCGCCGCTGCCTGCATCTGCAATTAACGGa                              <  2:1117699/68‑1 (MQ=255)                                 gTTGGATCGAACGTCGTGAAAGCGATAACGCTCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGt                       <  1:38869/68‑1 (MQ=255)                                                   aaaGCGATAACGCTCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAg     <  2:155137/68‑1 (MQ=255)                                                    aaGCGATAACGCTCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAgg    <  2:272585/68‑1 (MQ=255)                                                     aGCGATAACGCTCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGt   >  2:1169207/1‑68 (MQ=255)                                                      gCGATAACGCTCGCCGCTGCCTGCATCTGCAATTAACGGAAATAGGTCACGAGTTTTTGCGCGAGGtt  <  2:327093/68‑1 (MQ=255)                                                      gCGATAACGCTCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGtt  <  2:531779/68‑1 (MQ=255)                                                               |                                                           GACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTT  >  NC_000913/2811033‑2811153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑