Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,413,571	T→C	intergenic (‑106/‑293)	acrS ← / → acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,413,571	0	T	C	100.0%	17.0 / NA	6	intergenic (‑106/‑293)	acrS/acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/3);  total (3/3)

ATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTA  >  NC_000913/3413524‑3413622                                                |                                                    aTCTATCACCTAATGTGTATTTATACGAGATGCTAATATTGAGTTGCCATAAATCGTTAAataaataa                                 <  1:263820/68‑1 (MQ=255)          cTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATAtatta                        >  2:1104001/1‑68 (MQ=255)                                      atTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTa  >  1:857014/1‑62 (MQ=255)                                      atTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTa  <  2:857014/62‑1 (MQ=255)                                           gTTGCCATAAATCGTTAAATAAATAATATATATTATTTAc                   >  1:784541/1‑40 (MQ=255)                                           gTTGCCATAAATCGTTAAATAAATAATATATATTATTTAc                   <  2:784541/40‑1 (MQ=255)                                                |                                                    ATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTA  >  NC_000913/3413524‑3413622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑