| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,041,386 | 0 | G | C | 45.5% | 14.9 / 12.7 | 22 | G257A (GGA→GCA) | appA | phosphoanhydride phosphorylase |
| Reads supporting (aligned to +/- strand): ref base G (7/5); new base C (0/10); total (7/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.35e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACACCAGTGGAACACCTTGCTAAGTTTGCATAACGCGCAATTTTATTTGCTA > NC_000913/1041330‑1041453 | gCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCg > 1:488565/1‑68 (MQ=255) cTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGa < 1:348254/68‑1 (MQ=255) tGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGGGGGGAAGGATCACCGAt < 1:369391/68‑1 (MQ=255) cgACGGAGATATTTCTCCTGCAACATGCTCAGGGAATGCCGGAGCCGTGGTGGGCAATGATCACCGAt < 1:306155/67‑1 (MQ=255) ggAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTcac < 1:270796/68‑1 (MQ=255) gagaTATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGCAAGGATCACCGATTcaca < 1:645529/68‑1 (MQ=255) agaTATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGAt > 2:567304/1‑62 (MQ=255) agaTATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGAt < 1:567303/62‑1 (MQ=255) atatTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGCAAGGATCACCGATTCACACCa < 2:613179/68‑1 (MQ=255) atTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGTCAAGGATCACCGATTCACACCAGt < 2:74058/68‑1 (MQ=255) atTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACACCAGt > 2:562269/1‑68 (MQ=255) ctcCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACACCAGTGGag > 2:698578/1‑67 (MQ=255) cTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGCAAGGATCACCGATTCACACCAGTGGAacac < 1:403798/68‑1 (MQ=255) gCAACAAGCACTGGGATTGCCGGTGCCGGGGTGGGCAAGGATCAACGATTCACACCAGTGGAACACCt < 1:230086/68‑1 (MQ=255) gCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGCAAGGATCACCGATTCACACCAGTGGAACACCt < 1:511488/68‑1 (MQ=255) gCACTGGTAATGCCGTAGCCGGGGTGGGCAAGGATCACCGATTCACACCAGTGGAACACCTTGCTAAg < 2:488565/68‑1 (MQ=255) gCACAGGGAATGCCGGAGCCGGGGTGGGCAAGGATCACCGATTCACACCAGTGGAACACCTTGCTAAg < 1:405994/68‑1 (MQ=255) cacaGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACACCAGTGGAACACCTTGCTAAGt > 2:542855/1‑68 (MQ=255) gAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACACCAGTGGAACACCTTGCTAAGTTTGCAt > 2:508417/1‑68 (MQ=255) aTGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACACCAGTGGAACACCTTGCTAAGTTTGCATaa > 2:65668/1‑68 (MQ=255) ccGTAGCCGTGTTGTGGAAGTAGCACCGATTCTCATCTGTTGAACACCTTGCTAAGTTTGCATAAcgc < 2:487159/68‑1 (MQ=255) gCCGGGGTGGGCTAGGAGCACCGATTCACACCAGTGGAACACCTTGCTAAGTTTGCATAACGCGCAAt < 1:144635/68‑1 (MQ=255) gAAGGATCACCGATTCACACCAGTGGAACACCTTGCTAAGTTTGCATAACGCGCAATTTTATTTGCTa > 1:211034/1‑68 (MQ=255) | GCTGACGGAGATATTTCTCCTGCAACAAGCACAGGGAATGCCGGAGCCGGGGTGGGGAAGGATCACCGATTCACACCAGTGGAACACCTTGCTAAGTTTGCATAACGCGCAATTTTATTTGCTA > NC_000913/1041330‑1041453 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |