Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,114,419	G→A	A18A (GCC→GCT)	fpr ←	ferredoxin‑NADP reductase; flavodoxin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,114,419	0	G	A	100.0%	31.2 / NA	14	A18A (GCC→GCT)	fpr	ferredoxin‑NADP reductase; flavodoxin reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/7);  total (7/7)

TAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTC  >  NC_000913/4114364‑4114481                                                        |                                                               tAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGAGCGTCGGTCCAg                                                    >  2:148205/1‑68 (MQ=255)  aaaTTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGAGCGTCTGTCCAGt                                                   >  2:404252/1‑68 (MQ=255)  aaaTTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGAGCGTCGGTCCAGt                                                   <  2:108195/68‑1 (MQ=255)  aaaTTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGAGCGTCGGTCCAGt                                                   <  2:45261/68‑1 (MQ=255)     ttGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGAGCGTCGGTCCAGTTCt                                                >  1:694295/1‑68 (MQ=255)                  aaCGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGAGCGTCGGTCCAGTTCTGCACTTTAGTGAc                                   <  1:517809/68‑1 (MQ=255)                   aCGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGAGCGTCGGTCCAGTTCTGCACTTTAGTGACt                                  >  1:300025/1‑68 (MQ=255)                        aGCACGGGGGCGTGAACGGTGAGACTAAACAGAGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGcc                             <  2:300025/68‑1 (MQ=255)                         gCACGGGGGCGTGAACGGTGAGACTAAACAGAGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCt                            <  2:422457/68‑1 (MQ=255)                              ggggCGTGAACGGTGAGACTAAACAGAGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTATTAc                       <  2:642466/68‑1 (MQ=255)                                       aCGGTGAGACTAAACAGAGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTATTACCCAATCatc              <  2:272702/68‑3 (MQ=255)                                              gaCTAAACAGAGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTATTACCCAATCAGCCATGttt       >  2:406008/1‑68 (MQ=255)                                                   aaCAGAGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTATTACCCAATCAGCCATGTTTTtctc  >  2:623167/1‑68 (MQ=255)                                                   aaCAGAGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTATTACCCAATCAGCCATGTTTTtctc  >  2:623879/1‑68 (MQ=255)                                                        |                                                               TAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTC  >  NC_000913/4114364‑4114481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑