Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,312,590	G→A	Q54* (CAG→TAG)	ompC ←	outer membrane porin protein C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,312,590	0	G	A	100.0%	116.0 / NA	34	Q54* (CAG→TAG)	ompC	outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/20);  total (14/20)

AACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCT  >  NC_000913/2312530‑2312654                                                             |                                                                 aaCCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCa                                                           <  1:351776/68‑1 (MQ=255)  aCCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCAt                                                          <  1:60890/68‑1 (MQ=255)  aCCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCAt                                                          >  1:326301/1‑68 (MQ=255)  aCCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCAt                                                          >  2:630911/1‑68 (MQ=255)      gTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTAc                                                      <  2:313547/68‑1 (MQ=255)          gCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCt                                                  >  2:265159/1‑68 (MQ=255)              gTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTttgt                                              <  1:271558/68‑1 (MQ=255)                    aaCCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCag                                        >  2:167501/1‑68 (MQ=255)                    aaCCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCag                                        >  1:388509/1‑68 (MQ=255)                       cTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAgaga                                     >  1:13248/1‑68 (MQ=255)                        tGATTTTCACCTTTGAAGCCTAGACGCATTTATGTCTAGTCTCCTTCTTCATCTTTGTTGTCAGAGaa                                    <  2:655783/68‑1 (MQ=25)                        tGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGaa                                    <  1:167501/68‑1 (MQ=255)                            tctCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAg                                <  1:73005/66‑1 (MQ=255)                             ttCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGt                               <  1:255676/68‑1 (MQ=255)                                gccTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCa                            >  1:352389/2‑68 (MQ=255)                                   ttagAAGCCAAGACTCATGTTGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAAAAGTGCTGGc                         <  1:434786/65‑1 (MQ=255)                                      gAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGt                      <  2:13248/68‑1 (MQ=255)                                      gAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGt                      <  2:255704/68‑1 (MQ=255)                                      gAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGt                      <  1:99469/68‑1 (MQ=255)                                      gAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGt                      <  1:270227/68‑1 (MQ=255)                                        aGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCt                    <  2:543977/68‑1 (MQ=255)                                        aGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCt                    >  2:613307/1‑68 (MQ=255)                                        aGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCt                    >  2:614170/1‑68 (MQ=255)                                        aGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCt                    <  1:265159/68‑1 (MQ=255)                                        aGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCt                    >  2:658786/1‑68 (MQ=255)                                        aGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGCGCAGGCCGTCt                    <  1:285783/68‑1 (MQ=255)                                          ccAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTAc                  <  2:211083/68‑1 (MQ=255)                                                  cATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGt          <  2:224388/68‑1 (MQ=255)                                                       aGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGa     <  2:620151/68‑1 (MQ=255)                                                       aGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGa     >  1:76495/1‑68 (MQ=255)                                                       aGGGCTAGTCGCCATCTACATCTTTGTTGTCAGATAAATAGTGCAGGCCGTCTACTTTACCGTACAGa     <  2:551293/68‑1 (MQ=255)                                                        ggTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGAt    >  1:230203/1‑68 (MQ=255)                                                          tCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCt  >  1:253223/1‑68 (MQ=255)                                                          tCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCt  >  1:146824/1‑68 (MQ=255)                                                             |                                                                 AACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCT  >  NC_000913/2312530‑2312654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑