Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,679,525	G→T	A1074D (GCT→GAT)	yphG ←	DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,679,525	0	G	T	100.0%	67.2 / NA	21	A1074D (GCT→GAT)	yphG	DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/8);  total (13/8)

GTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTC  >  NC_000913/2679462‑2679577                                                                |                                                     gTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCgg                                                  >  1:396893/1‑68 (MQ=255)   ttAGTGAACGCGGTTAAAAATAAAAGGCATCTCGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGAt                                                >  2:396398/1‑68 (MQ=255)      gTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTaa                                             >  1:17260/1‑68 (MQ=255)       tGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAAt                                            >  1:70700/1‑68 (MQ=255)                         aaaGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGcgcg                          <  1:21331/68‑1 (MQ=255)                             gCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCa                      >  1:260348/1‑68 (MQ=255)                               aTCACGGTTGTTAATAATGCCGCTTTCGGCCATTCCGGATTATTCGCCGTCAATTCAGCTCGAGCAGa                    <  1:16650/68‑1 (MQ=255)                               aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                    <  1:37229/68‑1 (MQ=255)                               aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                    <  2:260348/68‑1 (MQ=255)                               aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGGCAATTCAGCGCGAGCAGa                    <  1:282221/68‑1 (MQ=255)                                 cACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                  <  1:31908/68‑1 (MQ=255)                                 cACGGCGGCGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                  <  1:512168/68‑1 (MQ=255)                                    ggtggtGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCat               >  2:151627/1‑68 (MQ=255)                                            ataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa       >  1:94123/1‑68 (MQ=255)                                            ataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa       >  1:415191/1‑68 (MQ=255)                                            ataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa       >  2:360278/1‑68 (MQ=255)                                              aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc     >  2:227194/1‑68 (MQ=255)                                               atGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAcc    >  2:205674/1‑68 (MQ=255)                                                 gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTc  >  2:198995/1‑68 (MQ=255)                                                 gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTc  <  1:35337/68‑1 (MQ=255)                                                 gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTc  >  2:73458/1‑68 (MQ=255)                                                                |                                                     GTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTC  >  NC_000913/2679462‑2679577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑