| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,312,212 | +TC | coding (538/1104 nt) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,312,210 | 1 | . | T | 96.5% | 82.2 / ‑3.1 | 29 | coding (540/1104 nt) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (13/15); total (13/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
| * | NC_000913 | 2,312,210 | 2 | . | C | 96.5% | 94.2 / ‑2.6 | 29 | coding (540/1104 nt) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (13/15); total (13/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
CGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCT‑‑TCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAA > NC_000913/2312143‑2312268 || cgccgACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCt < 1:570868/68‑1 (MQ=255)cgccgACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCt < 1:744462/68‑1 (MQ=255) aCGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCT‑‑Tctca < 1:1325387/68‑4 (MQ=255) tttGACGCTGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGc < 2:397269/68‑1 (MQ=255) gACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGt < 1:1279298/68‑1 (MQ=255) aCGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGtt < 2:1286452/68‑1 (MQ=255) aCGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGtt < 1:153797/68‑1 (MQ=255) gCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGtttt > 1:1342100/1‑68 (MQ=255) tGCGTCATGACCGTTGTTAGTTTCGCCACTAGTAAAGCCTTCTCACCAGTTGGGTTGCCGTTTTTAcg < 2:1458819/68‑2 (MQ=255) tGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTAcc > 1:270540/1‑68 (MQ=255) cACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTACCCTGGt < 1:1274537/68‑1 (MQ=255) cACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTACCCTGGt < 1:1355890/68‑1 (MQ=255) cACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTACCCTGGt < 1:424707/68‑1 (MQ=255) aCGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTACCCTggca > 1:604901/1‑66 (MQ=255) aCGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTACCCTGGTa > 2:1158549/1‑68 (MQ=255) aCGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTACCCTGGTa > 2:1313588/1‑68 (MQ=255) aCGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTAACCCTGGTa > 2:546275/1‑68 (MQ=255) gACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTACCCTGGTACt > 1:1176735/1‑68 (MQ=255) ccGTTGTTAGTTACGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTACCCTGGCACTGa < 2:604897/68‑1 (MQ=255) gttagttaCGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGc < 1:81585/68‑1 (MQ=38) ttagttaCGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACa > 2:1084040/1‑65 (MQ=38) ttagttaCGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACa < 1:1084044/65‑1 (MQ=38) gttaCGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAg < 2:1243268/68‑1 (MQ=38) ttaCGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGt < 2:1176731/68‑1 (MQ=37) ttaCGCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGt > 1:910136/1‑68 (MQ=37) aCGCCACTAGTAAAGCCTTCTCACAAGATGGGTTGCAGTTTTTACACTGGTACTGAACAGCAAAGTTc > 1:877295/1‑68 (MQ=25) gCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCg > 2:517279/1‑36 (MQ=25) gCCACTAGTAAAGCCTTCTCACCAGATGGGTTGCCg < 1:517282/36‑1 (MQ=25) cACTAGTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGc > 1:794693/1‑68 (MQ=25) gTAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTTCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCa < 2:388804/68‑1 (MQ=25) tAAAGCCTTCTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa > 2:507078/1‑68 (MQ=25) || CGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCT‑‑TCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAA > NC_000913/2312143‑2312268 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |