| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,563,398 | 0 | G | C | 34.6% | 30.1 / 15.5 | 27 | S779C (TCC→TGC) | dosP | oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator |
| Reads supporting (aligned to +/- strand): ref base G (11/6); new base C (0/9); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.41e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTG > NC_000913/1563331‑1563460 | ttGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCggg > 1:25473/1‑68 (MQ=255)ttGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGCAGGGGGCggg < 2:897609/68‑1 (MQ=255) tCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGaaa > 2:773899/1‑68 (MQ=255) aTTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAAGaa < 2:1460385/68‑1 (MQ=255) cAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAACAAATATc < 2:835863/68‑1 (MQ=255) gTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGa > 1:1443538/1‑68 (MQ=255) cacGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATaa > 1:1144171/1‑68 (MQ=255) cGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAAcg > 2:465494/1‑68 (MQ=255) gctgctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAAcgc > 1:74611/1‑68 (MQ=255) gctgctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGAGGGAAAAGAAATATCCCTGAATAAcga > 1:1137996/1‑67 (MQ=255) gctCATCCATCCTGGAATTTCTTCGGCGGGTAGGGGGCGGTAAAATAAATATCCCTGAATAACGCGAc < 1:576891/68‑1 (MQ=255) gctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGAc < 1:576893/68‑1 (MQ=255) gctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAAGAAATATCCCTGAATAACGCGAc < 1:1425781/68‑1 (MQ=255) gctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAACAAATATCCCTGAATAACGCGAc < 1:885140/68‑1 (MQ=255) tCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAAGAAATATCCCTGAATAACGCGACAg < 2:1436774/68‑1 (MQ=255) tCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAACAAATATCCCTGAATAACGCGACAg < 2:918593/68‑1 (MQ=255) cccAGCCTGGAATTTCTTCGGCGGTTAGGGGGCGGGAAATGAAATATCCCTGAATAACGCGACAGTgg < 2:1311050/67‑1 (MQ=255) ccAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGa < 1:1005851/68‑1 (MQ=255) cAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACTGTGGAt > 1:462620/1‑68 (MQ=255) gCCTGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAAGAAATATCCCTGAATAACGCGACAGCGGATCt < 2:683039/68‑1 (MQ=255) tgtaaTTCCTTCGGCGGGTAGGGGGCGTCAAAACAAATATCCCTGAATAACGCGACAGTGGATCTTGc < 1:1424809/65‑1 (MQ=255) aTTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAg > 1:111810/1‑68 (MQ=255) cttcttcGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGc < 2:1443533/67‑1 (MQ=255) tcttcGGCGGGTAGGGGGCGGCAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCAt < 1:543018/68‑1 (MQ=255) gTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGc < 2:25473/68‑1 (MQ=255) gggCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCttt < 1:36036/68‑1 (MQ=255) ggCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTg > 2:795473/1‑68 (MQ=255) ggCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTg > 2:1267487/1‑68 (MQ=255) ggCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTg > 2:944254/1‑68 (MQ=255) | TTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTG > NC_000913/1563331‑1563460 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |