| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,770,233 | 0 | C | G | 37.0% | 38.8 / 14.4 | 27 | intergenic (+87/+10) | yibU/yibH | pseudogene, H repeat‑associated protein/putative membrane fusion protein (MFP) component of efflux pump |
| Reads supporting (aligned to +/- strand): ref base C (2/15); new base G (10/0); total (12/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.82e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAGTAATATGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATCTCCCCCCTCCCCCTCAATGATCCAAATAAAGATAATGCATCCAGCTGGTCATTCTTAGCAGCACTTTCC > NC_000913/3770178‑3770298 | gAAGTAATATGCAAAGATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATCTGGGCCCTCCCCCTCa > 2:944993/1‑68 (MQ=255)gAAGTAATATGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATCTCCCCCCTCCCCCTCa < 1:950008/68‑1 (MQ=255) aGTAATATGCTAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATGTGAGACCTCCCCCTCaaa > 1:796790/1‑67 (MQ=255) aGTAATATGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTGGATAATGTGGGCCCGCCCCCTCAAt > 2:753386/1‑68 (MQ=255) aGTAATATGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCGGTAATGTGACGCCTCCCCCACaag > 2:994308/1‑67 (MQ=255) tAATATGCAAAGATAATTAGTCCTTACAAGGAGAAAAGTTCGAGAATCAGCGCCAGCACCCTCAATGa > 2:1248482/1‑68 (MQ=255) aaTATGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATGTGGGCCCACCCCCACAAAGag > 2:325225/1‑67 (MQ=255) atatGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATCTCCCCCCTCCCCCTCAATGATc < 1:753185/68‑1 (MQ=255) tatGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCAATAATGTGGGCCAACCCCCTCAAAGATca > 2:524609/1‑67 (MQ=255) atGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATGTGGGCCCTCCCCCACAAAGATCCa > 2:330752/1‑68 (MQ=255) aaaTAATTAGTCCTTACAAGGAGCAAAGTTCCATAATCTCCCCCCTCCCCCTCAATGATCCAAATaaa < 1:477418/68‑1 (MQ=255) aataatTAGTCCTTACAAGGAGCAAAGTTCCATAATCTCCCCCCTCCCCCTCAATGATCCAAATAAAg < 1:924479/68‑1 (MQ=255) taatTAGTCCTTACAAGGAGCAAAGTTCCATAATCTCCCCCCTCCCCCTCAATGATCCAAATAAAGAt < 2:1428044/68‑1 (MQ=255) tAGTCCTTACAAGGAGCAAAGTTCCATAATCTCCCCCCTCCCCCTCAATGATCCAAATAAAGATAATg < 2:916559/68‑1 (MQ=255) tCCTTACAAGGAGCAGAGTTGCATAATGAGACCCCTCCCCCACAAGGATCCAAATAAAGATAATGAAt > 2:1411384/1‑68 (MQ=255) ttACAAGGAGCAAAGTTCCATAATCTCCCCCCTTCCCCTCAATGATCCAAATAAAGATAATGCATCCa < 2:1356923/68‑1 (MQ=255) atACAAGGAGCAAAGTTCCATAATCTCCCCCCTCCCCCTCAATGATCCAAATAAAGATAATGCATCCa < 1:1353530/67‑1 (MQ=255) aCAAGGAGCAAAGTTCCATAATGTGGGAACACCCACACAAAGAACCAAATAAAGATAATGCATACAGc > 1:744507/1‑68 (MQ=255) cAAGGAGCAAAGTTCCATAATGTGGGCCCACCCCCACAATGGTCCAAATAAAGATAATGCAACCAGcg > 2:1160391/1‑67 (MQ=255) ggAGCAAAGTTCCATAATGTGGGCCCGCCCCCTCAATGATCCAAAAAAAGATAATGAAAACAGCAGGt > 1:676606/1‑68 (MQ=255) ggAGCAAAGTTCCATAATCTCCCCCCTCCCCCTCAATGATCCAAATAAAGATAATGCATCCAGCTGGt < 1:325227/68‑1 (MQ=255) gCAAAGTTCCATAATCTCCCCCCTCCCCCTCAATGATCCAAATAAAGATAATGCATCCAGCTGGTCAt < 1:889607/68‑1 (MQ=255) gCAAAGTTCCATAATCTCCCCCCTCCCCCTCAATGATCCAAATAAAGATAATGCATCCAGCTGGTCAt < 1:753390/68‑1 (MQ=255) aaaGTTCCATAATCTCCCCCCTCCCCCTCAATGATCCAAATAAAGATAATGCATCCAGCTGGTCATTc < 1:192675/68‑1 (MQ=255) aaGTTCCATAATCTCCCCCCTCCCCCTCAATGATCCAAATATAGATAATGCATCCAGCTGGTCATTCt < 2:393571/68‑1 (MQ=255) gTTCCATAATCTCCCCCCTCCCCCTCAATGATCCAAATAAAGATAATGCATCCAGCTGGTCATTCTTa < 2:1243473/68‑1 (MQ=255) cATAATCTCCCCCCTCCCCCTCAATGATCCAAATAAAGATAATGCATCCAGCTGGTCATTCTTagcag < 1:256779/68‑1 (MQ=255) cccccctcccccTCAATGATCCAAATAAAGATAATGCATCCAGCTGGTCATTCTTAGCAGCACTTTcc < 1:768413/68‑1 (MQ=255) | GAAGTAATATGCAAAAATAATTAGTCCTTACAAGGAGCAAAGTTCCATAATCTCCCCCCTCCCCCTCAATGATCCAAATAAAGATAATGCATCCAGCTGGTCATTCTTAGCAGCACTTTCC > NC_000913/3770178‑3770298 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |