Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	264,098	0	G	T	100.0%	104.2 / NA	31	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/12);  total (19/12)

GGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGT  >  NC_000913/264034‑264162                                                                 |                                                                 gggTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGGCCTGGCGGCGTTCGGCGTATACCGCTAgg                                                               <  2:83726/68‑1 (MQ=255)   gTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAggcc                                                             >  2:170651/1‑66 (MQ=255)        gCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCgg                                                        >  1:780009/1‑68 (MQ=255)             tcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGata                                                   >  2:1388401/1‑68 (MQ=255)             tcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGata                                                   >  1:642541/1‑68 (MQ=255)              caggcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACCCCGGCGatat                                                  <  2:814621/68‑1 (MQ=255)                  caggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCgg                                              >  2:247171/1‑68 (MQ=255)                  caggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCgg                                              >  1:589952/1‑68 (MQ=255)                  caggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCgg                                              >  1:83353/1‑68 (MQ=255)                      tataGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACg                                          <  1:1141630/68‑1 (MQ=255)                       ataGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGt                                         <  2:902683/68‑1 (MQ=255)                       ataGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGt                                         <  2:83352/68‑1 (MQ=255)                        taGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGata                                                   >  2:1260585/1‑57 (MQ=255)                        taGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGata                                                   <  1:1260589/57‑1 (MQ=255)                           ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCt                                     >  2:701415/1‑68 (MQ=255)                           ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCt                                     >  2:473547/1‑68 (MQ=255)                            gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                    >  2:838323/1‑68 (MQ=255)                            gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                    >  1:1171306/1‑68 (MQ=255)                            gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                    >  1:456470/1‑68 (MQ=255)                            gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                    >  2:1305975/1‑68 (MQ=255)                            gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                    >  2:1271167/1‑68 (MQ=255)                            gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                    >  1:1082222/1‑68 (MQ=255)                                 cttcGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt                               <  2:1237985/65‑1 (MQ=255)                                   gCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtgt                             >  2:116965/1‑68 (MQ=255)                                       tCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCTGAACTTCCCTGCCGGTGTAATg                         <  1:541285/68‑1 (MQ=18)                                       tCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATg                         <  2:830259/68‑1 (MQ=255)                                       tCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATg                         >  1:622178/1‑68 (MQ=255)                                        ccTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGa                        <  2:428138/68‑1 (MQ=255)                                                 gTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTa               <  2:714037/68‑1 (MQ=255)                                                      gCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTg          <  2:622174/68‑1 (MQ=255)                                                            aCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTg    >  1:1358611/1‑68 (MQ=255)                                                              cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGt  <  1:110355/68‑1 (MQ=255)                                                              cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGt  <  2:325085/68‑1 (MQ=255)                                                              cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGt  <  1:237585/68‑1 (MQ=255)                                                              cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGt  <  2:1125615/68‑1 (MQ=255)                                                                 |                                                                 GGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGT  >  NC_000913/264034‑264162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑