Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,197,502	C→T	G11R (GGA→AGA)	ymfD ←	e14 prophage; putative SAM‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,197,502	0	C	T	100.0%	144.2 / NA	45	G11R (GGA→AGA)	ymfD	e14 prophage; putative SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/30);  total (15/30)

CTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTT  >  NC_000913/1197437‑1197564                                                                  |                                                               cTCGCAAAGATATCTAGAGGGCATCGTATGAGTTTTTGCTTCATTCTCTGAGCGAATATTAACTCTGt                                                              <  1:523932/68‑1 (MQ=255) cTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                                              <  1:1099863/68‑1 (MQ=255) cTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                                              <  2:1168187/68‑1 (MQ=255) cTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                                              <  2:64796/68‑1 (MQ=255)  tCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCtgtg                                                             >  1:1010780/1‑68 (MQ=255)   cGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGc                                                            <  1:734089/68‑1 (MQ=255)   cGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGc                                                            <  1:1246767/68‑1 (MQ=255)   cGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGc                                                            <  1:350219/68‑1 (MQ=255)   cGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGc                                                            <  2:276713/68‑1 (MQ=255)   cGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGc                                                            <  2:396552/68‑1 (MQ=255)     cAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCat                                                          <  1:870945/68‑1 (MQ=255)     cAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCat                                                          <  1:955877/68‑1 (MQ=255)        aGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCAtatt                                                       <  2:584771/68‑1 (MQ=255)        aGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCAtatt                                                       >  2:136022/1‑68 (MQ=255)        aGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCAtatt                                                       <  1:1092086/68‑1 (MQ=255)        aGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCAtatt                                                       <  2:1263600/68‑1 (MQ=255)                agagGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                                              <  1:166299/53‑1 (MQ=255)                agagGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                                              >  2:1081521/1‑53 (MQ=255)                agagGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                                              >  2:166297/1‑53 (MQ=255)                agagGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                                              <  1:1081525/53‑1 (MQ=255)                agagGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCa                                               >  2:1012522/1‑68 (MQ=255)                  agGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                                              <  2:843993/51‑1 (MQ=255)                  agGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt                                                              >  1:843998/1‑51 (MQ=255)                  agGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCAtatt                                                       <  1:1406761/58‑1 (MQ=255)                  agGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCAtatt                                                       >  2:1406756/1‑58 (MQ=255)                  agGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCaaa                                             >  2:914951/1‑68 (MQ=255)                   gggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGc                                                            <  1:1336984/52‑1 (MQ=255)                   gggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGc                                                            >  2:1336980/1‑52 (MQ=255)                   gggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAg                                            <  1:301234/68‑1 (MQ=255)                   gggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAg                                            >  2:1118648/1‑68 (MQ=255)                   gggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAg                                            <  1:1372011/68‑1 (MQ=255)                   gggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAg                                            >  2:1281524/1‑68 (MQ=255)                    ggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGc                                           <  1:761111/68‑1 (MQ=255)                     gCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCa                                          >  2:1395413/1‑68 (MQ=255)                     gCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCa                                          >  1:785170/1‑68 (MQ=255)                        tCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGt                                       <  1:309688/68‑1 (MQ=255)                                ggTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCa                               >  2:1150453/1‑68 (MQ=255)                                ggTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCa                               >  1:532623/1‑68 (MQ=255)                                ggTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCa                               <  1:1408849/68‑1 (MQ=255)                                ggTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCa                               <  1:1395418/68‑1 (MQ=255)                                ggTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCa                               <  1:1012527/68‑1 (MQ=255)                                      gctgcATTCTCTGAGCGAATATTTACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAAt                         <  1:178144/68‑1 (MQ=255)                                      gctgcATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAAt                         <  1:265415/68‑1 (MQ=255)                                      gctgcATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAAt                         <  2:620641/68‑1 (MQ=255)                                        tgcATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGt                       <  1:248260/68‑1 (MQ=255)                                                             aaCTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCtt  >  1:398698/1‑68 (MQ=255)                                                                  |                                                               CTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTT  >  NC_000913/1197437‑1197564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑