Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,413,571	T→C	intergenic (‑106/‑293)	acrS ← / → acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,413,571	0	T	C	100.0%	52.2 / NA	15	intergenic (‑106/‑293)	acrS/acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/8);  total (7/8)

AAAATAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAATATAT  >  NC_000913/3413508‑3413633                                                                |                                                               aaaaTAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATaa                                                            >  2:1025692/1‑68 (MQ=255)                    tATCACCTAATGTGCATTTATACTAGAGGCTAATATTGAGTTGCCATAAATCGTTACATAAATAatat                                         <  2:593572/68‑1 (MQ=255)                    aaTCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAatat                                         <  2:152643/67‑1 (MQ=255)                        gccTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAatatatat                                     >  2:289757/2‑68 (MQ=255)                         ccTAAAGTGTATTTATACGAGAGGCTAATATTGAGTAGCCATAAATCGTTAAATAAATAATATAtatt                                    <  1:1102343/68‑1 (MQ=255)                          cTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATc                                                         <  1:1474938/46‑1 (MQ=255)                          cTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATc                                                         >  2:1474933/1‑46 (MQ=255)                                     ttATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGa                        <  1:1424485/68‑1 (MQ=255)                                        taCGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATAc                     >  2:1090027/1‑68 (MQ=255)                                                  tAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACa           <  1:1025697/68‑1 (MQ=255)                                                   aaTATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCa                <  1:548448/62‑1 (MQ=255)                                                   aaTATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCa                >  2:548445/1‑62 (MQ=255)                                                    atatTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATc         <  1:1090031/68‑1 (MQ=255)                                                        tGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAata     >  1:591799/1‑68 (MQ=255)                                                           gTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAatatat  >  1:375227/1‑68 (MQ=255)                                                                |                                                               AAAATAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAATATAT  >  NC_000913/3413508‑3413633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑