Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,413,571	T→C	intergenic (‑106/‑293)	acrS ← / → acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,413,571	0	T	C	100.0%	111.1 / NA	30	intergenic (‑106/‑293)	acrS/acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/16);  total (14/16)

ATAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAATATA  >  NC_000913/3413511‑3413632                                                             |                                                              aTAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATc                                                        <  2:551631/68‑1 (MQ=255)        aGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaat                                                 <  2:377319/68‑1 (MQ=255)         ggTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaata                                                >  1:239551/1‑68 (MQ=255)         ggTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaata                                                >  2:51082/1‑68 (MQ=255)         ggTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaata                                                >  2:51081/1‑68 (MQ=255)          gTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaataa                                               <  2:306137/68‑1 (MQ=255)             aaTCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAaataaata                                            <  1:735162/68‑1 (MQ=255)               tCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAaataat                                          <  1:531807/68‑1 (MQ=255)                cTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAataata                                         <  2:540749/68‑1 (MQ=255)                cTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAataata                                         >  2:396559/1‑68 (MQ=255)                           tgtgTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTa                              >  2:723277/1‑68 (MQ=255)                            gtgtATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTAc                             <  1:461130/68‑1 (MQ=255)                            gtgtATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTAc                             <  1:314218/68‑1 (MQ=255)                             tgtATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTTAATAAATAATATATATTATTTAcc                            >  2:563704/1‑68 (MQ=255)                                 tttATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAg                        >  2:655531/1‑68 (MQ=255)                                  ttATACGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGa                       <  2:56302/68‑1 (MQ=255)                                     taCGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATAc                    >  2:3186/1‑68 (MQ=255)                                              cTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTAc           <  1:51082/68‑1 (MQ=255)                                              cTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTAc           <  1:51081/68‑1 (MQ=255)                                               tAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACa          <  1:2508/68‑1 (MQ=255)                                                 atatTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATc        <  2:181740/68‑1 (MQ=255)                                                 atatTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATc        <  2:394365/68‑1 (MQ=255)                                                   atTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCaa      >  2:533014/1‑68 (MQ=255)                                                   atTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCaa      >  2:429450/1‑68 (MQ=255)                                                    ttGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAat     >  1:659658/1‑68 (MQ=255)                                                      gAGTTGCCATAAATCGTTAAATAAATAAAATATATTATTTACCTAAGATACATTCACTACATCAatat   <  2:383369/68‑1 (MQ=255)                                                       aGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAatata  >  1:347770/1‑68 (MQ=255)                                                       aGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAatata  >  1:346866/1‑68 (MQ=255)                                                        gTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCa               >  1:390263/1‑54 (MQ=255)                                                        gTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCa               <  2:390264/54‑1 (MQ=255)                                                             |                                                              ATAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAATATA  >  NC_000913/3413511‑3413632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑