Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	264,098	0	G	T	100.0%	64.6 / NA	20	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/9);  total (11/9)

GGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGT  >  NC_000913/264035‑264162                                                                |                                                                 ggTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGa                                                              <  2:559833/68‑1 (MQ=255)  gTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAgggc                                                             >  1:44779/1‑66 (MQ=255)  gTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc                                                             >  1:345323/1‑68 (MQ=255)  gTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc                                                             >  2:221205/1‑68 (MQ=255)   tGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACt                                                            <  2:610630/68‑1 (MQ=255)        cTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGc                                                       <  2:246544/68‑1 (MQ=255)            tcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGata                                                   >  1:269073/1‑68 (MQ=255)                 caggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACAGCTAGGACTCCGGCGATATCCgg                                              >  2:24479/1‑68 (MQ=255)                          ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCt                                     >  1:174216/1‑68 (MQ=255)                          ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCt                                     >  1:528396/1‑68 (MQ=255)                          ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCt                                     >  1:151755/1‑68 (MQ=255)                           gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTg                                    >  2:653853/1‑68 (MQ=255)                                ttGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt                               <  2:345324/68‑1 (MQ=255)                                 tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                              <  2:269073/68‑1 (MQ=255)                                     gTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAAt                          >  1:536672/1‑68 (MQ=255)                                      tCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGGTATCCGGAACGTCCCTGCCGGTGTAATg                         >  1:369758/1‑68 (MQ=21)                                       ccTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGa                        <  1:653852/68‑1 (MQ=255)                                                gTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTa               <  2:98669/68‑1 (MQ=255)                                                 ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTAc              <  2:151755/68‑1 (MQ=255)                                                       gTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGcc        <  1:304630/68‑1 (MQ=255)                                                             cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGt  <  2:714701/68‑1 (MQ=255)                                                             cgcgAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGt  <  2:549070/68‑1 (MQ=255)                                                                |                                                                 GGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGT  >  NC_000913/264035‑264162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑