| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,197,502 | C→T | G11R (GGA→AGA) | ymfD ← | e14 prophage; putative SAM‑dependent methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,197,502 | 0 | C | T | 92.9% | 71.2 / NA | 28 | G11R (GGA→AGA) | ymfD | e14 prophage; putative SAM‑dependent methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (9/17); minor base A (1/1); total (10/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCT > NC_000913/1197437‑1197563 | cTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt > 2:750926/1‑68 (MQ=255)cTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt < 1:722063/68‑1 (MQ=255)cTCGCAAAGATATCTAGAGGGCATCGTACGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGt < 1:540253/68‑1 (MQ=255) cGCAAAGATATCTAGAGTGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGc < 2:688014/68‑1 (MQ=255) cGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGc < 1:590716/68‑1 (MQ=255) cAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCat < 2:406305/68‑1 (MQ=255) cAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCat < 1:210571/68‑1 (MQ=255) cAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCat < 2:247584/68‑1 (MQ=255) cAAAGATATCTAGAGGGCATCGTATGAGGTTTCGCTGCATTCTCTGAGCGAATATTAACTCTGTGCat < 2:467154/68‑1 (MQ=255) aGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCAtatt < 1:289239/68‑1 (MQ=255) tCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGt < 2:546029/68‑1 (MQ=255) agagGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCa > 1:289472/1‑68 (MQ=255) agagGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCa > 2:645804/1‑68 (MQ=255) gggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAg < 2:535213/68‑1 (MQ=255) gggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAg < 2:445070/68‑1 (MQ=255) tgCATCTTAGTAGGTTTTTCTGCATTCTATGATCGAATATTAACTCTGTGCTTTTTATAGTTCAAAGc < 1:168193/67‑1 (MQ=255) ggCAACGAATGAGGTTTTGCTGCATTCTATTCGCGAATAAAAACTCAGACCATATAATAGTTCAAAGc > 2:168193/1‑68 (MQ=255) aTGAGGTTTTGCATCATTCTCTGATCGAATATTAACTCAGTTCATATTATAGTTCAAAGCAAGTACCa < 2:213986/68‑1 (MQ=255) ggTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCa < 1:470228/68‑1 (MQ=255) ggTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCa > 1:727192/1‑68 (MQ=255) gTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCAtatt < 2:136392/43‑1 (MQ=255) gTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCAtatt > 1:136392/1‑43 (MQ=255) gTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCaa > 1:308616/1‑68 (MQ=255) ctgcATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATg > 2:279183/1‑68 (MQ=255) tctcTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTAttt > 1:235407/1‑68 (MQ=255) gCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGa < 1:280930/68‑1 (MQ=255) cGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGaa > 2:306759/1‑68 (MQ=255) tAACTCTGTTCATATTATAGTTCAAAGCAAGTACCATTCTATTAATGTTATTTTTAGTGAAAAATTCt < 1:102671/68‑1 (MQ=255) | CTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCT > NC_000913/1197437‑1197563 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |